PUBLICATION
Cloning and Characterization of Replication Protein A p32 Complementary DNA in Zebrafish ( Danio rerio)
- Authors
- Lee, J.S., and Lee, Y.S
- ID
- ZDB-PUB-040216-7
- Date
- 2002
- Source
- Marine biotechnology (New York, N.Y.) 4(1): 1-5 (Journal)
- Registered Authors
- Lee, Jae-Seong
- Keywords
- none
- MeSH Terms
- none
- PubMed
- 14961282 Full text @ Mar. Biotechnol.
Citation
Lee, J.S., and Lee, Y.S (2002) Cloning and Characterization of Replication Protein A p32 Complementary DNA in Zebrafish ( Danio rerio). Marine biotechnology (New York, N.Y.). 4(1):1-5.
Abstract
Replication protein A (RPA) is a heterotrimeric single-stranded DNA-binding protein (70, 32, and 14 kDa) that is an essential component of the DNA replication fork. A complementary DNA encoding zebrafish RPA 32-kDa subunit was isolated by screening a zebrafish embryo lambdaZAPII cDNA library with a human RPA p32 cDNA probe. The zebrafish RPA p32 cDNA consisted of 1097 bp encoding 272 amino acid residues. The deduced amino acid sequence shows high similarity to mouse and human RPA p32. In vitro phosphorylation of zebrafish RPA protein by Cdc2 kinase was shown. A recombinant protein of zebrafish RPA p32 containing a short histidine tag at the NH(2)-terminus was overexpressed in Escherichia coli BL21(DE3) pLys using an inducible T7 expression system, and was purified by Ni-NTA affinity chromatography. In this article, cloning of the zebrafish RPA p32 cDNA is reported in relation to the study of DNA replication in fish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping