PUBLICATION
T cells and the thymus in developing zebrafish
- Authors
- Danilova, N., Hohman, V.S., Sacher, F., Ota, T., Willett, C.E., and Steiner, L.A.
- ID
- ZDB-PUB-040216-26
- Date
- 2004
- Source
- Developmental and comparative immunology 28(7-8): 755-767 (Journal)
- Registered Authors
- Danilova, Nadia, Hohman, Valerie, Ota, Tatsuya, Steiner, Lisa, Willett, Catherine
- Keywords
- Zebrafish, Thymus, T cells, Development, TCR, V families
- MeSH Terms
-
- Embryo, Nonmammalian/metabolism
- Homeodomain Proteins/metabolism
- Animals
- Gene Expression Regulation, Developmental/genetics
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/metabolism*
- Spleen/embryology
- Spleen/metabolism*
- Amino Acid Sequence
- Intestines/embryology
- Intestines/metabolism*
- T-Lymphocytes/metabolism*
- Molecular Sequence Data
- Receptors, Antigen, T-Cell/metabolism
- In Situ Hybridization
- Thymus Gland/embryology
- Thymus Gland/metabolism*
- PubMed
- 15043944 Full text @ Dev. Comp. Immunol.
Citation
Danilova, N., Hohman, V.S., Sacher, F., Ota, T., Willett, C.E., and Steiner, L.A. (2004) T cells and the thymus in developing zebrafish. Developmental and comparative immunology. 28(7-8):755-767.
Abstract
The expression of genes encoding T cell receptor (TCR) was used to follow the development of the thymus and to analyze the distribution of T cells in zebrafish. In the thymus, expression was first detected, by in situ hybridization, at four days post fertilization. In RNA extracted from whole fish, TCR transcripts were also detected at four days and reached adult levels at three weeks. At six weeks, TCR was expressed throughout the thymus, whereas rag1 expression was localized to the peripheral regions. Expression of TCR outside the thymus was detected at nine days. In adult peripheral organs, the greatest expression was in the pronephros, mesonephros and intestine; expression in the spleen became greater as fish age. Three new, relatively highly expressed, TCR V families were identified.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping