PUBLICATION
Early requirement for fgf8 function during hindbrain pattern formation in zebrafish
- Authors
- Wiellette, E.L., and Sive, H.
- ID
- ZDB-PUB-040204-2
- Date
- 2004
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 229(2): 393-399 (Journal)
- Registered Authors
- Sive, Hazel, Wiellette, Elizabeth
- Keywords
- fibroblast growth factor, fgf3, fgf8, zebrafish, hindbrain, valentino, krox20, rhombomere, neural patterning
- MeSH Terms
-
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/metabolism
- Body Patterning/drug effects
- Body Patterning/physiology
- Somites/drug effects
- Somites/metabolism
- Animals
- Fibroblast Growth Factor 8
- Gene Expression Regulation, Developmental/drug effects
- Rhombencephalon/embryology*
- Rhombencephalon/metabolism*
- In Situ Hybridization
- Fibroblast Growth Factors/antagonists & inhibitors
- Fibroblast Growth Factors/metabolism*
- Oligonucleotides, Antisense/pharmacology
- Morphogenesis/drug effects
- Morphogenesis/physiology
- Zebrafish/embryology*
- Zebrafish/metabolism
- Fibroblast Growth Factor 3
- Signal Transduction/drug effects
- Signal Transduction/physiology
- Zebrafish Proteins/metabolism
- PubMed
- 14745965 Full text @ Dev. Dyn.
Citation
Wiellette, E.L., and Sive, H. (2004) Early requirement for fgf8 function during hindbrain pattern formation in zebrafish. Developmental Dynamics : an official publication of the American Association of Anatomists. 229(2):393-399.
Abstract
Fibroblast growth factor (FGF) signaling is required for normal development of the vertebrate brain, including the isthmus and caudal regions of the hindbrain. Recent work in zebrafish has identified a requirement for the combination of fgf3 and fgf8 functions in specification of rhombomeres 5 and 6 (r5, r6), when evaluated at mid- and late somitogenesis stages. However, when examined earlier in development, during early somitogenesis stages, FGF8 alone is required to initiate r5 and r6 development. Both a mutation in fgf8 and injection of fgf8-targeted antisense morpholino-modified oligonucleotides result in suppression of genes normally expressed in r5 and r6 by the one- to two-somite stage. This expression recovers by the six-somite stage, and we propose that this recovery is a response to activation of fgf3 and to delayed accumulation of fgf8. These data demonstrate an early, nonredundant requirement for fgf8 function in hindbrain patterning. Developmental Dynamics 229:393-399, 2004. Copyright 2004 Wiley-Liss, Inc.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping