PUBLICATION
Mouse MafA, homologue of zebrafish somite Maf 1, contributes to the specific transcriptional activity through the insulin promoter
- Authors
- Kajihara, M., Sone, H., Amemiya, M., Katoh, Y., Isogai, M., Shimano, H., Yamada, N., and Takahashi, S.
- ID
- ZDB-PUB-040113-6
- Date
- 2003
- Source
- Biochemical and Biophysical Research Communications 312(3): 831-842 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Xenopus
- Chickens
- Promoter Regions, Genetic/genetics
- Sensitivity and Specificity
- Humans
- Sequence Alignment
- Mice, Inbred C57BL
- Sequence Homology, Amino Acid
- Transcription Factors/chemistry*
- Transcription Factors/genetics
- Transcription Factors/metabolism*
- Lectins, C-Type
- Maf Transcription Factors, Large
- Organ Specificity
- Animals
- Saccharomyces cerevisiae Proteins/chemistry*
- Saccharomyces cerevisiae Proteins/genetics
- Saccharomyces cerevisiae Proteins/metabolism*
- Molecular Sequence Data
- Amino Acid Sequence
- Proto-Oncogene Proteins/chemistry*
- Proto-Oncogene Proteins/genetics
- Proto-Oncogene Proteins/metabolism*
- Somites/chemistry
- Somites/metabolism
- Transcriptional Activation/physiology*
- Zebrafish
- Tissue Distribution
- Trans-Activators/chemistry*
- Trans-Activators/genetics
- Trans-Activators/metabolism*
- Homeodomain Proteins*
- Mice
- Sequence Analysis, Protein
- Insulin/genetics*
- Insulin/metabolism*
- PubMed
- 14680841 Full text @ Biochem. Biophys. Res. Commun.
Citation
Kajihara, M., Sone, H., Amemiya, M., Katoh, Y., Isogai, M., Shimano, H., Yamada, N., and Takahashi, S. (2003) Mouse MafA, homologue of zebrafish somite Maf 1, contributes to the specific transcriptional activity through the insulin promoter. Biochemical and Biophysical Research Communications. 312(3):831-842.
Abstract
Large Maf transcription factors, which are members of the basic leucine zipper (b-Zip) superfamily, have been reported to be involved in embryonic development and cell differentiation. Previously, we isolated a novel zebrafish large Maf cDNA, somite Maf1 (SMaf1), which possesses transactivational activity within its N-terminus domain. To elucidate SMaf1 function in mammals, we tried to isolate the mouse homologue of zebrafish SMaf1. We isolated the mouse homologue of zebrafish SMaf1, which is the same molecule as the recently reported MafA. MafA mRNA was detected in formed somites, head neural tube, and liver cells in the embryos. In the adult mouse, MafA transcript was amplified in the brain, lung, spleen, and kidney by RT-PCR. MafA mRNA was also detectable in beta-cell line. Next, we analyzed the transcriptional activity of MafA using rat insulin promoters I and II (RIPI and II), since a part of RIP sequence was similar to the Maf recognition element (MARE) and MafA was expressed in pancreatic beta cells. MafA was able to activate transcription from RIPII, but not RIPI, in a dose dependent manner and the activity was dependent on RIPE3b/C1 sequences. In addition, the amount of MafA protein was regulated by glucose concentration. These results indicate that MafA is the homologue of zebrafish SMaf1 and acts as a transcriptional activator of the insulin gene promoter through the RIPE3b element.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping