PUBLICATION
Identification of 5(')-upstream region of pufferfish ribosomal protein L29 gene as a strong constitutive promoter to drive GFP expression in zebrafish
- Authors
- Chang, M.H., Chou, C.M., Hsieh, Y.C., Lu, I.C., Devi, M.K., Chang, J.P., Kuo, T.F., and Huang, C.J.
- ID
- ZDB-PUB-040113-2
- Date
- 2004
- Source
- Biochemical and Biophysical Research Communications 314(1): 9-258 (Other)
- Registered Authors
- Huang, Chang-Jen
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- COS Cells
- Caenorhabditis elegans
- Drosophila
- Gene Expression Regulation*
- Green Fluorescent Proteins
- Humans
- Luminescent Proteins/genetics*
- Luminescent Proteins/metabolism*
- Molecular Sequence Data
- Promoter Regions, Genetic*
- Recombinant Fusion Proteins/chemistry
- Recombinant Fusion Proteins/genetics
- Recombinant Fusion Proteins/metabolism
- Ribosomal Proteins/chemistry*
- Ribosomal Proteins/genetics*
- Ribosomal Proteins/metabolism
- Sequence Alignment
- Sequence Analysis, Protein*
- Sequence Homology, Amino Acid
- Species Specificity
- Structure-Activity Relationship
- Tetraodontiformes
- Transcriptional Activation
- Zebrafish
- PubMed
- 14715273 Full text @ Biochem. Biophys. Res. Commun.
Citation
Chang, M.H., Chou, C.M., Hsieh, Y.C., Lu, I.C., Devi, M.K., Chang, J.P., Kuo, T.F., and Huang, C.J. (2004) Identification of 5(')-upstream region of pufferfish ribosomal protein L29 gene as a strong constitutive promoter to drive GFP expression in zebrafish. Biochemical and Biophysical Research Communications. 314(1):9-258.
Abstract
The genomic structure of Tetraodon fluviatilis L29 gene was determined and its promoter activity was analyzed in COS-1 cells and zebrafish embryos. The TfL29 gene comprises four exons and three introns, spanning approximately 1.7kb. The 5(')-upstream 2.2-kb of the first exon contains 10 E-boxes and many putative binding motifs for transcription factors GATA-1, AML-1a, c-Myb, Oct-1, CdxA, and NRF-2. Promoter activity assay showed that the distal 2.2-kb fragment not only had high luciferase activity in COS-1 cells, but also strong and ubiquitous GFP expression in a variety of tissues in zebrafish embryos. On the other hand, there are no TATA or CAAT boxes within a 300-bp region upstream from the transcription initiation site. Although this region has high luciferase activity in COS-1 cells, it is not sufficient to drive GFP expression in zebrafish embryos. In this proximal 300-bp region, there are two E-boxes, two CdxA sites, and one NRF-2 site that is immediately downstream of the transcription start site.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping