PUBLICATION
A novel smoothelin-like, actin-binding protein required for choroidal fissure closure in zebrafish
- Authors
- Kurita, R., Tabata, Y., Sagara, H., Arai, K., and Watanabe, S.
- ID
- ZDB-PUB-040109-14
- Date
- 2004
- Source
- Biochemical and Biophysical Research Communications 313(4): 1092-1100 (Journal)
- Registered Authors
- Watanabe, Sumiko
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Blood Vessels/embryology
- Choroid/embryology*
- Cloning, Molecular
- Cytoskeletal Proteins/genetics
- DNA, Complementary/genetics
- Gene Expression Regulation, Developmental/drug effects
- Microfilament Proteins/genetics*
- Molecular Sequence Data
- Muscle Proteins/genetics
- Oligoribonucleotides, Antisense/genetics
- Oligoribonucleotides, Antisense/pharmacology
- Optic Nerve/embryology
- Retina/embryology
- Sequence Homology, Amino Acid
- Zebrafish/embryology*
- Zebrafish/genetics*
- Zebrafish Proteins/genetics*
- PubMed
- 14706655 Full text @ Biochem. Biophys. Res. Commun.
Citation
Kurita, R., Tabata, Y., Sagara, H., Arai, K., and Watanabe, S. (2004) A novel smoothelin-like, actin-binding protein required for choroidal fissure closure in zebrafish. Biochemical and Biophysical Research Communications. 313(4):1092-1100.
Abstract
A gene expressed in the choroidal fissure of the zebrafish eye was isolated. This gene, designated #61, contained significant homology with the previously reported actin-binding protein smoothelin. During zebrafish embryogenesis, #61 expression was first detected in the lateral mesoderm of the mid-trunk region, and then strong expression was observed in the choroid fissure of the eye and in a part of the brain at 30hpf. Abrogation of #61 activity by an antisense morpholino oligonucleotide resulted in the failure of closure of the choroid fissure at 30hpf. In addition, hemorrhage was observed at the caudal side of the eye. Detailed analysis indicated that leakage of blood may have arisen from the hyaloid vessels and the primordial midbrain channels. On the other hand, retinal differentiation and optic nerve formation seemed normal. Taken together, our data suggest that gene #61 may play a role in the formation of hyaloid vessels and subsequent choroid fissure closure.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping