ZFIN ID: ZDB-PUB-031229-9
Whole-cell patch-clamp recordings from identified spinal neurons in the zebrafish embryo
Saint-Amant, L. and Drapeau, P.
Date: 2003
Source: Methods in cell science : an official journal of the Society for In Vitro Biology   25(1-2): 59-64 (Review)
Registered Authors: Drapeau, Pierre, Saint-Amant, Louis
Keywords: embryo, locomotion, motor behavior, neurogenesis, patch-clamp, spinal cord
MeSH Terms:
  • Animals
  • Electrophysiology
  • Embryo, Nonmammalian/physiology
  • Neurons/physiology*
  • Patch-Clamp Techniques*
  • Spinal Cord/physiology*
  • Zebrafish/embryology
  • Zebrafish/physiology*
PubMed: 14739588 Full text @ Methods Cell Sci.
ABSTRACT
We describe a preparation for obtaining patch-clamp recordings from identified embryonic spinal cord interneurons, motoneurons and sensory neurons in an in vivo zebrafish preparation. This preparation is used to study the spatial and temporal patterns of spontaneous and touch-evoked electrical activity during the initial development of circuitry in the spinal cord. The combination of these physiological techniques with the powerful genetic and molecular tools available in the zebrafish has the potential to increase our understanding of the complex interactions between genes and electrical activity during the development of the vertebrate nervous system.
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