PUBLICATION
Whole-cell patch-clamp recordings from identified spinal neurons in the zebrafish embryo
- Authors
- Saint-Amant, L. and Drapeau, P.
- ID
- ZDB-PUB-031229-9
- Date
- 2003
- Source
- Methods in cell science : an official journal of the Society for In Vitro Biology 25(1-2): 59-64 (Review)
- Registered Authors
- Drapeau, Pierre, Saint-Amant, Louis
- Keywords
- embryo, locomotion, motor behavior, neurogenesis, patch-clamp, spinal cord
- MeSH Terms
-
- Animals
- Electrophysiology
- Embryo, Nonmammalian/physiology
- Neurons/physiology*
- Patch-Clamp Techniques*
- Spinal Cord/physiology*
- Zebrafish/embryology
- Zebrafish/physiology*
- PubMed
- 14739588 Full text @ Methods Cell Sci.
Citation
Saint-Amant, L. and Drapeau, P. (2003) Whole-cell patch-clamp recordings from identified spinal neurons in the zebrafish embryo. Methods in cell science : an official journal of the Society for In Vitro Biology. 25(1-2):59-64.
Abstract
We describe a preparation for obtaining patch-clamp recordings from identified embryonic spinal cord interneurons, motoneurons and sensory neurons in an in vivo zebrafish preparation. This preparation is used to study the spatial and temporal patterns of spontaneous and touch-evoked electrical activity during the initial development of circuitry in the spinal cord. The combination of these physiological techniques with the powerful genetic and molecular tools available in the zebrafish has the potential to increase our understanding of the complex interactions between genes and electrical activity during the development of the vertebrate nervous system.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping