PUBLICATION
Developmental differences in susceptibility to neomycin-induced hair cell death in the lateral line neuromasts of zebrafish (Danio rerio)
- Authors
- Murakami, S.L., Cunningham, L.L., Werner, L.A., Bauer, E., Pujol, R., Raible, D.W., and Rubel, E.W.
- ID
- ZDB-PUB-031204-10
- Date
- 2003
- Source
- Hearing Research 186(1-2): 47-56 (Journal)
- Registered Authors
- Raible, David
- Keywords
- Ototoxicity; Critical period; Apoptosis; Hearing loss; Aminoglycoside; Deafness
- MeSH Terms
-
- Animals
- Analysis of Variance
- Anti-Bacterial Agents/toxicity*
- Dose-Response Relationship, Drug
- Critical Period, Psychological
- Microscopy, Electron
- Cell Death
- Neomycin/toxicity*
- Hair Cells, Auditory/drug effects*
- Hair Cells, Auditory/physiology
- Hair Cells, Auditory/ultrastructure
- Immunohistochemistry
- Hearing Loss/chemically induced
- Protein Synthesis Inhibitors/toxicity*
- Zebrafish
- PubMed
- 14644458 Full text @ Hear. Res.
Citation
Murakami, S.L., Cunningham, L.L., Werner, L.A., Bauer, E., Pujol, R., Raible, D.W., and Rubel, E.W. (2003) Developmental differences in susceptibility to neomycin-induced hair cell death in the lateral line neuromasts of zebrafish (Danio rerio). Hearing Research. 186(1-2):47-56.
Abstract
Mechanosensory hair cells of the inner ear are susceptible to death when exposed to a variety of drugs including aminoglycoside antibiotics. During avian and mammalian development, there is a period of relative insensitivity to aminoglycoside-induced hair cell death. This study was designed to test the hypothesis that zebrafish (Danio rerio) have developmental differences in sensitivity to aminoglycoside-induced hair cell death in the lateral line neuromasts. Larval zebrafish of various ages were exposed to several concentrations of neomycin, and their hair cells were examined using the potentiometric vital dye, DASPEI. Results indicate that zebrafish larvae aged 4 days post-fertilization are relatively insensitive to aminoglycoside-induced hair cell death compared to older fish. Thus zebrafish hair cells show developmental differences in sensitivity to aminoglycoside-induced death similar to those reported for inner ear hair cells of birds and mammals.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping