PUBLICATION
Analysis of zebrafish cyp19 promoters
- Authors
- Tong, S.K. and Chung, B.
- ID
- ZDB-PUB-031119-1
- Date
- 2003
- Source
- Journal of steroid biochemistry 86(3-5): 381-386 (Journal)
- Registered Authors
- Chung, Bon-chu
- Keywords
- Ovary; Brain; Promoter; Aromatase; cyp19; P450aro; Zebrafish
- MeSH Terms
-
- Transcription Factors/metabolism
- Promoter Regions, Genetic/genetics
- Molecular Sequence Data
- Binding Sites
- Zebrafish/genetics*
- Zebrafish/metabolism*
- Aromatase/genetics*
- Aromatase/metabolism
- Humans
- Zebrafish Proteins
- DNA-Binding Proteins
- Tissue Distribution
- Animals
- Receptors, Cytoplasmic and Nuclear
- Steroidogenic Factor 1
- Base Sequence
- PubMed
- 14623534 Full text @ J. Steroid Biochem.
Citation
Tong, S.K. and Chung, B. (2003) Analysis of zebrafish cyp19 promoters. Journal of steroid biochemistry. 86(3-5):381-386.
Abstract
Cyp19 encodes P450 aromatase, the key enzyme catalyzing the conversion of androgens into estrogens. Estrogens play a crucial role in the anatomical, functional and behavioral characteristics of sexually dimorphic development. In zebrafish, two cyp19 genes, cyp19a and cyp19b, expressed in ovary and brain, respectively, were found. We have isolated the promoter regions of the zebrafish cyp19 genes from a bacterial artificial chromosome library to search for regulatory sequences that bind to transcription factors. Sequences like arylhydrocarbon receptor (AhR) recognition site, estrogen receptor recognition half sites (1/2ERE) and c-AMP responsive elements were found in the 5'-flanking regions of both cyp19 genes. For ovarian-specific expression, we found binding sites for steroidogenic factor-1 (SF-1), GATA transcription factor 4 (GATA-4) and Wilm tumor 1 (WT1-KTS) on the promoter region of cyp19a but not cyp19b. For brain-specific expression of the cyp19b gene, sequences for recognition of chicken ovalbumin upstream promoter-transcription factor (COUP) and Ptx-1 were detected in the promoter. The importance of these putative control elements in ovary and brain-specific promoter has been assessed by sequence comparison among various species.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping