ZFIN ID: ZDB-PUB-031105-3
Proximal upstream region of zebrafish bone morphogenetic protein 4 promoter directs heart expression of green fluorescent protein
Shentu, H., Wen, H.-J., Mour Her, G., Huang, C.-J., Wu, J.-L., and Hwang, S.-P.
Date: 2003
Source: Genesis (New York, N.Y. : 2000)   37(3): 103-112 (Journal)
Registered Authors: Huang, Chang-Jen, Hwang, Sheng-Ping L., Wu, Jen-Leih
Keywords: none
MeSH Terms:
  • Animals
  • Bone Morphogenetic Protein 4
  • Bone Morphogenetic Proteins/genetics*
  • Embryonic Development
  • Gene Expression Regulation, Developmental*
  • Green Fluorescent Proteins
  • Heart/physiology
  • Indicators and Reagents/analysis
  • Luminescent Proteins/analysis
  • Luminescent Proteins/biosynthesis*
  • Promoter Regions, Genetic*
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish Proteins
PubMed: 14595833 Full text @ Genesis
ABSTRACT
We examined the activity of the bone morphogenetic protein 4 (BMP4) promoter in zebrafish embryos via transient and stable transgenic expression analyses in order to obtain a better understanding of the regulation of BMP4 tissue-specific expression. Transient expression studies showed that the 9.0-kb BMP4 promoter/upstream region drove green fluorescent protein (GFP) expression mainly in the heart. Deletion analyses indicated the existence of multiple regulatory elements in the 7.5-kb BMP4 promoter/proximal upstream region. In addition, a coinjection experiment further demonstrated the 2.4-kb Bgl II-Hind III DNA region contains major positive regulatory elements. In addition, stable transgenic lines were established to further confirm the heart-specificity of this segment in BMP4 promoter. The results showed that GFP was mainly localized in the myocardium of developing ventricles of 48-hpf (hours postfertilization), 72-hpf, and 100-hpf transgenic F(1) embryos. Together, these results indicate that the 7.5-kb BMP4 promoter/proximal upstream region specifically contains regulatory elements for BMP4 expression in the heart, while regulatory elements for other endogenous BMP4-expressing tissues may reside in more distal regions and/or in introns.
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