The microRNA-producing enzyme Dicer1 is essential for zebrafish development
- Wienholds, E., Koudijs, M.J., van Eeden, F.J., Cuppen, E., and Plasterk, R.H.
- Nature Genetics 35(3): 217-218 (Journal)
- Registered Authors
- Cuppen, Edwin, Plasterk, Ronald H.A., van Eeden, Freek, Wienholds, Erno
- MeSH Terms
- DEAD-box RNA Helicases
- Gene Silencing
- RNA Helicases/genetics
- RNA Helicases/physiology*
- Ribonuclease III
- 14528306 Full text @ Nat. Genet.
Wienholds, E., Koudijs, M.J., van Eeden, F.J., Cuppen, E., and Plasterk, R.H. (2003) The microRNA-producing enzyme Dicer1 is essential for zebrafish development. Nature Genetics. 35(3):217-218.
MicroRNAs (miRNAs) are produced by the Dicer1 enzyme; the role of Dicer1 in vertebrate development is unknown. Here we report target-selected inactivation of the dicer1 gene in zebrafish. We observed an initial build-up of miRNA levels, produced by maternal Dicer1, in homozygous dicer1 mutants, but miRNA accumulation stopped after a few days. This resulted in developmental arrest around day 10. These results indicate that miRNA-producing Dicer1 is essential for vertebrate development.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes