PUBLICATION

Temporal and molecular separation of the kit receptor tyrosine kinase's roles in zebrafish melanocyte migration and survival

Authors
Rawls, J.F. and Johnson, S.L.
ID
ZDB-PUB-031001-8
Date
2003
Source
Developmental Biology   262(1): 152-161 (Journal)
Registered Authors
Johnson, Stephen L., Rawls, John F.
Keywords
none
MeSH Terms
  • Animals
  • Cell Survival
  • Melanocytes/physiology*
  • Oncogene Proteins/genetics
  • Oncogene Proteins/physiology*
  • Alleles
  • Zebrafish
  • Proto-Oncogene Proteins c-kit
  • Cell Movement
(all 9)
PubMed
14512025 Full text @ Dev. Biol.
Abstract
The Kit receptor tyrosine kinase is required by vertebrate melanocytes for their migration and survival. The relationship between these developmental roles of Kit, however, remains poorly understood. Here, we use two genetic approaches to demonstrate that Kit's roles in the migration and survival of embryonic melanocytes in the zebrafish (Danio rerio) are temporally and functionally independent. We use a temperature-sensitive kit mutation to show that kit promotes melanocyte migration and survival during distinct stages of development. These experiments additionally reveal that melanocyte migration is neither necessary nor sufficient for subsequent survival. We also identify kit alleles that molecularly separate kits roles in migration and survival. These results suggest that the melanocyte changes its response to Kit receptor signaling and function during development, first to promote migration, then to promote survival through distinct Kit-dependent mechanisms.
Genes / Markers
Figures
Figure Gallery (2 images)
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b5
    		Small Deletion
    j1e60
      		Point Mutation
      j1e78
        		Point Mutation
        j1e89
          		Point Mutation
          j1e99
            		Point Mutation
            j1e177
              		Point Mutation
              j1e249
                		Point Mutation
                1 - 7 of 7
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                Human Disease / Model
                No data available
                Sequence Targeting Reagents
                No data available
                Fish
                Fish
                kitab5/b5 (AB)
                kitaj1e60/j1e60
                kitaj1e78
                kitaj1e89/j1e89
                kitaj1e99/j1e99
                kitaj1e177/j1e177
                kitaj1e249/j1e249
                1 - 7 of 7
                Show
                Antibodies
                No data available
                Orthology
                No data available
                Engineered Foreign Genes
                No data available
                Mapping
                No data available
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                Citation
                Rawls, J.F. and Johnson, S.L. (2003) Temporal and molecular separation of the kit receptor tyrosine kinase's roles in zebrafish melanocyte migration and survival. Developmental Biology. 262(1):152-161.