PUBLICATION
Identification of Nogo-66 receptor (NgR) and homologous genes in fish
- Authors
- Klinger, M., Taylor, J.S., Oertle, T., Schwab, M.E., Stürmer, C.A., and Diekmann, H.
- ID
- ZDB-PUB-030902-10
- Date
- 2004
- Source
- Mol. Biol. Evol. 21(1): 76-85 (Journal)
- Registered Authors
- Diekmann, Heike, Klinger, Michael, Stuermer, Claudia, Taylor, John
- Keywords
- none
- MeSH Terms
-
- Animals
- Axons/metabolism
- Base Sequence
- Blotting, Southern
- Databases, Nucleic Acid
- Gene Components
- Gene Expression Regulation, Developmental*
- Genes, Duplicate/genetics
- Molecular Sequence Data
- Myelin Proteins/metabolism*
- Phylogeny*
- Radiation Hybrid Mapping
- Receptors, Cell Surface/genetics*
- Receptors, Cell Surface/metabolism
- Reverse Transcriptase Polymerase Chain Reaction
- Sequence Alignment
- Sequence Analysis, DNA
- Synteny/genetics
- Takifugu/genetics*
- Zebrafish/genetics*
- PubMed
- 12949137 Full text @ Mol. Biol. Evol.
Citation
Klinger, M., Taylor, J.S., Oertle, T., Schwab, M.E., Stürmer, C.A., and Diekmann, H. (2004) Identification of Nogo-66 receptor (NgR) and homologous genes in fish. Mol. Biol. Evol.. 21(1):76-85.
Abstract
The Nogo-66 receptor NgR has been implicated in the mediation of inhibitory effects of central nervous system (CNS) myelin on axon growth in the adult mammalian CNS. NgR binds to several myelin-associated ligands (Nogo-66, myelin associated glycoprotein, oligodendrocyte-myelin glycoprotein) which - among other inhibitory proteins - impair axonal regeneration in the CNS of adult mammals. In contrast to mammals, severed axons readily regenerate in the fish CNS. Nevertheless, fish axons are repelled by mammalian oligodendrocytes in vitro. Therefore, the identification of fish NgR homologs is a crucial step towards understanding NgR functions in vertebrate systems competent of CNS regeneration. Here, we report the discovery of four zebrafish (Danio rerio) and five fugu (Takifugu rubripes) NgR homologs. Synteny between fish and human, comparable intron-exon structures and phylogenetic analyses provide convincing evidence that the true fish orthologs were identified. The topology of the phylogenetic trees shows that the extra fish genes were produced by duplication events that occurred in ray-finned fishes prior to the divergence of the zebrafish and pufferfish lineages. Expression of zebrafish NgR homologs was detected relatively early in development and prominently in the adult brain suggesting functions in axon growth, guidance or plasticity.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping