ZFIN ID: ZDB-PUB-030826-10
Activity and distribution of paxillin, focal adhesion kinase, and cadherin indicate cooperative roles during zebrafish morphogenesis
Crawford, B.D., Henry, C.A., Clason, T.A., Becker, A.L., and Hille, M.B.
Date: 2003
Source: Molecular biology of the cell   14(8): 3065-3081 (Journal)
Registered Authors: Crawford, Bryan D., Henry, Clarissa A., Hille, Merrill
Keywords: none
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Cadherins/metabolism*
  • Cell Adhesion/physiology
  • Cloning, Molecular
  • Cytoskeletal Proteins/genetics
  • Cytoskeletal Proteins/metabolism*
  • Embryo, Nonmammalian/embryology
  • Embryo, Nonmammalian/metabolism
  • Fibronectins/metabolism
  • Focal Adhesion Protein-Tyrosine Kinases
  • Laminin/metabolism
  • Molecular Sequence Data
  • Morphogenesis/physiology
  • Notochord/embryology
  • Notochord/metabolism
  • Paxillin
  • Phosphoproteins/genetics
  • Phosphoproteins/metabolism*
  • Phosphorylation
  • Protein-Tyrosine Kinases/genetics
  • Protein-Tyrosine Kinases/metabolism*
  • Somites/cytology
  • Somites/metabolism
  • Zebrafish/embryology
  • Zebrafish/metabolism*
  • Zebrafish Proteins
PubMed: 12925747 Full text @ Mol. Biol. Cell
ABSTRACT
We investigated the focal adhesion proteins paxillin and Fak, and the cell-cell adhesion protein cadherin in developing zebrafish (Danio rerio) embryos. Cadherins are expressed in presomitic mesoderm where they delineate cells. The initiation of somite formation coincides with an increase in the phosphorylation of Fak, and the accumulation of Fak, phosphorylated Fak, paxillin, and fibronectin at nascent somite boundaries. In the notochord, cadherins are expressed on cells during intercalation, and phosphorylated Fak accumulates in circumferential rings where the notochord cells contact laminin in the perichordal sheath. Subsequently, changes in the orientations of collagen fibers in the sheath suggest that Fak-mediated adhesion allows longitudinal expansion of the notochord, but not lateral expansion, resulting in notochord elongation. Novel observations showed that focal adhesion kinase and paxillin concentrate at sites of cell-cell adhesion in the epithelial enveloping layer and may associate with actin cytoskeleton at epithelial junctions containing cadherins. Fak is phosphorylated at these epithelial junctions but is not phosphorylated on Tyr397, implicating a noncanonical mechanism of regulation. These data suggest that Fak and paxillin may function in the integration of cadherin-based and integrin-based cell adhesion during the morphogenesis of the early zebrafish embryo.
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