PUBLICATION
Molecular cloning of flounder Xp18, a newly identified highly conserved protein mainly expressed in the ovary
- Authors
- Cachot, J., Bultelle, F., Drouot, L., Galgani, F., Frebourg, T., Leboulenger, F., and Danger, J.M.
- ID
- ZDB-PUB-030815-3
- Date
- 2003
- Source
- Gene 307: 13-21 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Cloning, Molecular
- Conserved Sequence/genetics
- DNA, Complementary/chemistry
- DNA, Complementary/genetics
- Female
- Fish Proteins/genetics
- Flounder/genetics*
- Gene Expression
- Male
- Membrane Proteins/genetics*
- Molecular Sequence Data
- Ovary/metabolism*
- Phylogeny
- Sequence Alignment
- Sequence Analysis, DNA
- Sequence Homology, Amino Acid
- PubMed
- 12706884 Full text @ Gene
Citation
Cachot, J., Bultelle, F., Drouot, L., Galgani, F., Frebourg, T., Leboulenger, F., and Danger, J.M. (2003) Molecular cloning of flounder Xp18, a newly identified highly conserved protein mainly expressed in the ovary. Gene. 307:13-21.
Abstract
Screening of a flounder ovary cDNA library with a rainbow trout p53 probe led to the isolation of a p53-unrelated cDNA encoding an unknown 161 amino acid protein. In view of its apparent molecular weight and yet unknown function, the deduced protein was named Xp18. Corresponding orthologous cDNAs or expressed sequence tags have been identified in several species including human, rodents, bovine, chicken and zebrafish and a related cDNA has also been isolated in the fruit fly. Deduced amino acid sequences appeared to be extremely well conserved throughout vertebrate evolution. Structure predictions suggested that Xp18 may correspond to an integral protein comprising four transmembrane domains. The charged C-termini of all known vertebrate Xp18-like proteins displayed a characteristic KXKXX motif which is considered as an endoplasmic reticulum targeting sequence. Gene expression, as shown by Northern blot and quantitative reverse transcription-polymerase chain reaction analysis, was significantly higher in the ovary and to a lesser extent in the brain. Xp18 transcripts were also detected by in situ hybridization in most of the circumventricular regions of the brain of adult flounders. The gene encoding the human protein is located on chromosome Xq22.1, a genome region involved in numerous genetic diseases including premature ovarian failure.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping