PUBLICATION

Expression of a novel type I keratin, DAPK-1 in the dorsal aorta and pronephric duct of the zebrafish embryos

Authors
Jang, W.S., Kim, E.J., Ro, H., Kim, K.E., Huh, T.L., Kim, C.-H., and Rhee, M.
ID
ZDB-PUB-030812-2
Date
2003
Source
Gene   312: 145-150 (Journal)
Registered Authors
Huh, Tae-Lin, Kim, Cheol-Hee
Keywords
zebrafish (Danio rerio); Type I cytokeratin; Dorsal aorta; Pronephric duct
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Aorta/embryology
  • Aorta/metabolism*
  • DNA, Complementary/chemistry
  • DNA, Complementary/genetics
  • DNA, Complementary/isolation & purification
  • Embryo, Nonmammalian/metabolism*
  • Embryonic Development
  • Gene Expression Regulation, Developmental
  • Keratins/genetics*
  • Kidney/embryology
  • Kidney/metabolism*
  • Molecular Sequence Data
  • Mutation
  • Phylogeny
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish Proteins/genetics*
PubMed
12909350 Full text @ Gene
Abstract
We isolated a novel cytokeratin gene of zebrafish (Danio rerio), DAPK-1 closely related to other vertebrate type I cytokeratin genes. Zygotic transcription starts at the sphere stage. After the mid-blastula stage, DAPK-1 is expressed in all surface cells, notably in those of the outer enveloping layer. DAPK-1 messages are also present specifically during the segmentation, pharyngula, and hatching periods. In particular, after 24 h post-fertilization, its expression is restricted to the developing eye region, otic vesicle, pectoral fin, dorsal aorta, and pronephric duct. In the mindbomb mutant embryo that has defects in the dorsal aorta development, DAPK-1 transcripts are not detected in the dorsal aorta and pronephric duct. The characteristic expression pattern of DAPK-1 may facilitate more detailed studies related to the morphogenesis of dorsal aorta and pronephric duct.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes