PUBLICATION
Structure and characterization of the murine p80 coilin gene, Coil
- Authors
- Tucker, K.E., Massello, L.K., Gao, L., Barber, T.J., Hebert, M.D., Chan, E.K., and Matera, A.G.
- ID
- ZDB-PUB-030806-3
- Date
- 2000
- Source
- Journal of structural biology 129(2-3): 269-277 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Genomic Library
- Cloning, Molecular
- Recombinant Proteins/biosynthesis
- Recombinant Proteins/chemistry
- Brain/metabolism
- Sequence Homology, Nucleic Acid
- Sequence Alignment
- Animals
- Amino Acid Sequence
- Mice
- Base Sequence
- Sequence Homology, Amino Acid
- Exons
- Ranidae
- Nuclear Proteins/chemistry
- Nuclear Proteins/genetics*
- Humans
- Chromosome Mapping*
- Molecular Sequence Data
- Coiled Bodies/genetics
- Coiled Bodies/ultrastructure
- Phosphoproteins/genetics
- PubMed
- 10806077 Full text @ J. Struct. Biol.
Citation
Tucker, K.E., Massello, L.K., Gao, L., Barber, T.J., Hebert, M.D., Chan, E.K., and Matera, A.G. (2000) Structure and characterization of the murine p80 coilin gene, Coil. Journal of structural biology. 129(2-3):269-277.
Abstract
Cajal bodies (coiled bodies, CBs) are nuclear organelles of unknown function and are characterized by a wide variety of components including various basal transcription and cell cycle proteins, the nucleolar proteins fibrillarin and Nopp140, numerous small nuclear ribonucleoproteins, the survival motor neuron protein complex, and the marker protein, p80 coilin. To gain insight into the role of p80 coilin in CBs, we have cloned the murine gene Coil and have mapped it to the distal portion of chromosome band 11D. The approximately 2.6-kb transcript is detectable in all tissues analyzed, with the highest levels in brain and testis. Sequence analysis shows that, like its human counterpart, the mouse coilin gene is composed of seven exons and spans nearly 30 kb of genomic DNA. The predicted amino acid sequence reveals two conserved N- and C-terminal domains, and comparison with the Xenopus SPH-1 protein reveals that these three genes are indeed orthologous. These results should facilitate gene disruption experiments aimed at creating a genetic model system to study CBs.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping