PUBLICATION

An interacting network of T-box genes directs gene expression and fate in the zebrafish mesoderm

Authors
Goering, L.M., Hoshijima, K., Hug, B., Bisgrove, B., Kispert, A., and Grunwald, D.J.
ID
ZDB-PUB-030730-12
Date
2003
Source
Proceedings of the National Academy of Sciences of the United States of America   100(16): 9410-9415 (Journal)
Registered Authors
Bisgrove, Brent, Goering, Lisa, Grunwald, David, Hoshijima, Kazuyuki, Hug, Barbara
Keywords
none
MeSH Terms
  • Animals
  • Base Sequence
  • Fetal Proteins
  • Gene Expression Regulation, Developmental*
  • Genotype
  • Humans
  • Mesoderm/metabolism*
  • Models, Biological
  • Models, Genetic
  • Molecular Sequence Data
  • MyoD Protein/metabolism
  • Plasmids/metabolism
  • T-Box Domain Proteins/genetics*
  • T-Box Domain Proteins/metabolism
  • T-Box Domain Proteins/physiology*
  • Transcriptional Activation
  • Zebrafish
  • Zebrafish Proteins/metabolism
PubMed
12883008 Full text @ Proc. Natl. Acad. Sci. USA
Abstract
T-box genes encode transcription factors that play critical roles in generating the vertebrate body plan. In many developmental fields, multiple T-box genes are expressed in overlapping domains, establishing broad regions in which different combinations of T-box genes are coexpressed. Here we demonstrate that three T-box genes expressed in the zebrafish mesoderm, no tail, spadetail, and tbx6, operate as a network of interacting genes to regulate region-specific gene expression and developmental fate. Loss-of-function and gain-of-function genetic analyses reveal three kinds of interactions among the T-box genes: combinatorial interactions that generate new regulatory functions, additive contributions to common developmental pathways, and competitive antagonism governing downstream gene expression. We propose that T-box genes, like Hox genes, often function within gene networks comprised of related family members.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping