PUBLICATION
phospholipase C gamma-1 is required downstream of vascular endothelial growth factor during arterial development
- Authors
- Lawson, N.D., Mugford, J.W., Diamond, B.A., and Weinstein, B.M.
- ID
- ZDB-PUB-030717-17
- Date
- 2003
- Source
- Genes & Development 17(11): 1346-1351 (Journal)
- Registered Authors
- Diamond, Brianne, Lawson, Nathan, Mugford, Joshua, Weinstein, Brant M.
- Keywords
- none
- MeSH Terms
-
- Recombinant Proteins/metabolism
- Arteries/cytology
- Arteries/embryology*
- Crosses, Genetic
- Embryo, Nonmammalian/physiology
- Type C Phospholipases/genetics*
- Type C Phospholipases/metabolism
- Polymerase Chain Reaction
- Animals, Genetically Modified
- DNA Primers
- Muscle, Smooth, Vascular/cytology
- Muscle, Smooth, Vascular/embryology
- Zebrafish/embryology*
- Animals
- Base Sequence
- Vascular Endothelial Growth Factor A
- Genetic Carrier Screening
- Male
- Intercellular Signaling Peptides and Proteins/genetics*
- RNA, Messenger/genetics
- Lymphokines/genetics*
- Vascular Endothelial Growth Factors
- Female
- Endothelial Growth Factors/genetics*
- Molecular Sequence Data
- Gene Expression Regulation, Developmental*
- Phospholipase C gamma
- PubMed
- 12782653 Full text @ Genes & Dev.
Citation
Lawson, N.D., Mugford, J.W., Diamond, B.A., and Weinstein, B.M. (2003) phospholipase C gamma-1 is required downstream of vascular endothelial growth factor during arterial development. Genes & Development. 17(11):1346-1351.
Abstract
In this study, we utilize transgenic zebrafish with fluorescently labeled blood vessels to identify and characterize a mutant (y10) that displays specific defects in the formation of arteries, but not veins. We find that y10 encodes phospholipase C gamma-1 (plcg1), a known effector of receptor tyrosine kinase signaling. We further show that plcg1y10 mutant embryos fail to respond to exogenous Vegf. Our results indicate that Plcg1 functions specifically downstream of the Vegf receptor during embryonic development to govern formation of the arterial system.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping