PUBLICATION
Metabolism of bisphenol A in zebrafish (Danio rerio) and rainbow trout (Oncorhynchus mykiss) in relation to estrogenic response
- Authors
- Lindholst, C., Wynne, P.M., Marriott, P., Pedersen, S.N., and Bjerregaard, P.
- ID
- ZDB-PUB-030716-9
- Date
- 2003
- Source
- Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 135(2): 169-177 (Journal)
- Registered Authors
- Bjerregaard, Poul, Lindholst, Christian
- Keywords
- none
- MeSH Terms
-
- Animals
- Benzhydryl Compounds
- Bile/metabolism
- Estrogens/metabolism*
- Female
- Half-Life
- Liver/metabolism
- Male
- Oncorhynchus mykiss/metabolism*
- Phenols/blood
- Phenols/metabolism
- Phenols/pharmacokinetics*
- Phenols/toxicity
- Zebrafish/metabolism*
- PubMed
- 12860056 Full text @ Comp. Biochem. Physiol. C Toxicol. Pharmacol.
Citation
Lindholst, C., Wynne, P.M., Marriott, P., Pedersen, S.N., and Bjerregaard, P. (2003) Metabolism of bisphenol A in zebrafish (Danio rerio) and rainbow trout (Oncorhynchus mykiss) in relation to estrogenic response. Comparative biochemistry and physiology. Toxicology & pharmacology : CBP. 135(2):169-177.
Abstract
The kinetics of bisphenol A (BPA) were investigated in zebrafish (Danio rerio) exposed to 100 microg BPA/l. BPA uptake was measured during a 7-day period followed by an elimination phase of similar duration. After 2, 6, 12, 24, 48, 72, 120 and 168 h of uptake/elimination, fish were analysed for their content of BPA, bisphenol A glucuronic acid (BPAGA) and bisphenol A sulfate (BPAS). Within the first 24 h steady state levels of BPA, BPAGA and BPAS were reached and the total body concentrations were calculated to be 569, 12600 and 39.9 ng/g fish, respectively. Elimination rates of the three compounds in zebrafish were estimated by fitting the data to a compartment model. An initial rapid elimination phase was observed for BPA and BPAS with total body half lives (T(1/2)) of <1.1 h and 30 min, followed by a slower second elimination phase with T(1/2) values of 139 and 71 h, respectively. Excretion of BPAGA occurred from a single compartment with a T(1/2) of 35 h. The steady state concentration of BPA and its metabolites were investigated in rainbow trout (Oncorhynchus mykiss) exposed to 100 microg BPA/l. The toxicokinetic parameters from zebrafish and rainbow trout were compared; including previously published data on the rainbow trout. The data indicate that the smaller estrogenic sensitivity observed for the zebrafish may be caused by a more rapid metabolism of BPA in the zebrafish liver.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping