PUBLICATION
The expression of novel membrane-type matrix metalloproteinase isoforms is required for normal development of zebrafish embryos
- Authors
- Zhang, J., Bai, S., Zhang, X., Nagase, H., and Sarras, M.P.
- ID
- ZDB-PUB-030716-5
- Date
- 2003
- Source
- Matrix biology : journal of the International Society for Matrix Biology 22(3): 279-293 (Journal)
- Registered Authors
- Sarras, Michael P., Jr.
- Keywords
- none
- MeSH Terms
-
- Molecular Sequence Data
- Sequence Homology, Amino Acid
- Cell Membrane/enzymology
- Amino Acid Sequence
- Metalloendopeptidases/genetics
- Metalloendopeptidases/metabolism*
- In Situ Hybridization
- Matrix Metalloproteinases, Membrane-Associated
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism*
- Animals
- Base Sequence
- Humans
- Gene Expression Regulation, Enzymologic
- Genes, Reporter
- DNA, Complementary/genetics
- Isoenzymes/genetics
- Isoenzymes/metabolism
- Species Specificity
- Gene Expression Regulation, Developmental
- PubMed
- 12853038 Full text @ Matrix Biol.
Citation
Zhang, J., Bai, S., Zhang, X., Nagase, H., and Sarras, M.P. (2003) The expression of novel membrane-type matrix metalloproteinase isoforms is required for normal development of zebrafish embryos. Matrix biology : journal of the International Society for Matrix Biology. 22(3):279-293.
Abstract
Matrix metalloproteinases (MMPs) play important roles in the turnover of components of extracellular matrix (ECM) and in the processing of active and latent-signaling molecules bound to the ECM or associated with the cell surface. Through such actions, MMPs regulate a variety of cellular and developmental processes. Membrane-type matrix metalloproteinases (MT-MMPs) are of particular importance because they function in the immediate pericellular environment that modulates both cell-cell and cell-ECM interactions. In this study, we utilized zebrafish as a developmental model to study the role of MT-MMPs during early embryogenesis. We successfully isolated two isoforms of a MT-MMP homologue that are structurally similar to MT1-MMP. They have been named zebrafish MT-MMPalpha and beta. Zebrafish MT-MMPbeta is unique among vertebrate MT-MMPs in that it contains an Arg-Glu-Asp (RED) multiple- repeat motif in its linker region. Whole mount in situ analysis, RT-PCR , immunofluorescence, reporter analysis, Western blot analysis, and zymography indicated that MT-MMPalpha and beta were expressed through at least the first 72 h of development and that this expression was targeted to the cell surface. Functional studies using injection of either mRNA or morpholino antisense oligonucleotides resulted in a truncation of the cranial to caudal axis as monitored through 72 h post fertilization, indicating that zebrafish MT-MMPalpha and beta had an important role in embryonic development. Axis markers indicated that these effects likely involved processes occurring later than 10 h of embryogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping