PUBLICATION
Identification, classification, and partial characterization of genes in humans and other vertebrates homologous to a fish membrane progestin receptor
- Authors
- Zhu, Y., Bond, J., and Thomas, P.
- ID
- ZDB-PUB-030715-10
- Date
- 2003
- Source
- Proceedings of the National Academy of Sciences of the United States of America 100(5): 2237-2242 (Journal)
- Registered Authors
- Zhu, Yong
- Keywords
- none
- MeSH Terms
-
- Amino Acids/chemistry
- Animals
- Blotting, Northern
- Cell Membrane/metabolism
- Cytoplasm/metabolism
- DNA, Complementary/metabolism
- Escherichia coli/metabolism
- Humans
- Kinetics
- Mice
- Molecular Sequence Data
- Nucleic Acid Hybridization
- Phylogeny
- Progesterone/pharmacology
- Protein Binding
- Protein Structure, Secondary
- RNA, Messenger/metabolism
- Receptors, Progesterone/chemistry*
- Receptors, Progesterone/genetics*
- Recombinant Proteins/metabolism
- Species Specificity
- Steroids/metabolism
- Swine
- Takifugu
- Time Factors
- Tissue Distribution
- Xenopus
- Zebrafish
- PubMed
- 12601167 Full text @ Proc. Natl. Acad. Sci. USA
Citation
Zhu, Y., Bond, J., and Thomas, P. (2003) Identification, classification, and partial characterization of genes in humans and other vertebrates homologous to a fish membrane progestin receptor. Proceedings of the National Academy of Sciences of the United States of America. 100(5):2237-2242.
Abstract
Recently we discovered a previously uncharacterized gene with the characteristics of a membrane progestin receptor (mPR) in a fish model, spotted seatrout. Here, we report the identification, cloning, and characteristics of other members of this hitherto unknown family of putative mPRs from several vertebrate species, including human, mouse, pig, Xenopus, zebrafish, and Fugu, with highly conserved nucleotide and deduced amino acid sequences and similar structures to the spotted seatrout mPR. The 13 vertebrate genes identified seem to belong to an unknown gene family. Phylogenetic analysis indicates these cDNAs comprise three distinct groups (named alpha, beta, and gamma) within this gene family. Structural analyses of the translated cDNAs suggest they encode membrane proteins with seven transmembrane domains. The transcript sizes of the human alpha, beta, and gamma putative mPR mRNAs varied from 2.8 to 5.8 kb and showed distinct distributions in reproductive, neural, kidney and intestinal tissues, respectively. Recombinant human alpha, gamma, and mouse beta proteins produced in an Escherichia coli expression system demonstrated high affinity (K(d) = 20-30 nM) saturable binding for progesterone. Further analysis of binding to the gamma-subtype revealed binding was specific for progestins and was displaceable, with rapid rates of association and dissociation (t(1/2) = 2-8 min). These results suggest this is a new family of steroid receptors unrelated to nuclear steroid receptors, but instead having characteristics of G protein-coupled receptors.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping