PUBLICATION
vhnf1 and Fgf signals synergize to specify rhombomere identity in the zebrafish hindbrain
- Authors
- Wiellette, E.L. and Sive, H.
- ID
- ZDB-PUB-030707-10
- Date
- 2003
- Source
- Development (Cambridge, England) 130(16): 3821-3829 (Journal)
- Registered Authors
- Sive, Hazel, Wiellette, Elizabeth
- Keywords
- Vhnf1, Fibroblast growth factor, Fgf3, Fgf8, Zebrafish, Hindbrain, Valentino, Krox20, Rhombomere, Neural patterning
- MeSH Terms
-
- Animals
- DNA-Binding Proteins/genetics
- DNA-Binding Proteins/metabolism*
- Epistasis, Genetic
- Fibroblast Growth Factors/genetics
- Fibroblast Growth Factors/metabolism*
- Gene Expression Regulation, Developmental*
- Hepatocyte Nuclear Factor 1-beta
- Homeodomain Proteins/genetics
- Homeodomain Proteins/metabolism
- In Situ Hybridization
- MafB Transcription Factor
- Morphogenesis
- Nerve Tissue Proteins/genetics
- Nerve Tissue Proteins/metabolism
- Nuclear Proteins/genetics
- Nuclear Proteins/metabolism*
- Oligonucleotides, Antisense/genetics
- Oligonucleotides, Antisense/metabolism
- Rhombencephalon/anatomy & histology
- Rhombencephalon/embryology*
- Rhombencephalon/physiology
- Signal Transduction/physiology*
- Transcription Factors/genetics
- Transcription Factors/metabolism*
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism
- Zebrafish Proteins*
- PubMed
- 12835397 Full text @ Development
Citation
Wiellette, E.L. and Sive, H. (2003) vhnf1 and Fgf signals synergize to specify rhombomere identity in the zebrafish hindbrain. Development (Cambridge, England). 130(16):3821-3829.
Abstract
Vertebrate hindbrain segmentation is a highly conserved process but the mechanism of rhombomere determination is not well understood. Recent work in the zebrafish has shown a requirement for fibroblast growth factor (Fgf) signaling and for the transcription factor variant hepatocyte nuclear factor 1 (vhnf1) in specification of rhombomeres 5 and 6 (r5+r6). We show here that vhnf1 functions in two ways to subdivide the zebrafish caudal hindbrain domain (r4-r7) into individual rhombomeres. First, vhnf1 promotes r5+r6 identity through an obligate synergy with Fgf signals to activate valentino and krox20 expression. Second, vhnf1 functions independently of Fgf signals to repress hoxb1a expression. Although vhnf1 is expressed in a broad posterior domain during gastrulation, it promotes the specification of individual rhombomeres. This is achieved in part because vhnf1 gives cellular competence to respond to Fgf signals in a caudal hindbrain-specific manner.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping