ZFIN ID: ZDB-PUB-030702-3
The zebrafish fgf24 mutant identifies an additional level of Fgf signaling involved in vertebrate forelimb initiation
Fischer, S., Draper, B.W., and Neumann, C.J.
Date: 2003
Source: Development (Cambridge, England)   130(15): 3515-3524 (Journal)
Registered Authors: Draper, Bruce, Neumann, Carl J.
Keywords: Zebrafish, Limb development, fgf24, fgf10, tbx5, wnt2b, ikarus, Pectoral fin, Apical ectodermal ridge
MeSH Terms:
  • Animals
  • Cell Movement/physiology
  • Embryonic Induction/genetics
  • Embryonic Induction/physiology*
  • Fibroblast Growth Factors/genetics*
  • Fibroblast Growth Factors/metabolism
  • Forelimb/embryology*
  • Growth Substances/metabolism
  • Limb Buds/metabolism
  • Mutation
  • T-Box Domain Proteins/metabolism
  • Zebrafish/embryology*
  • Zebrafish/genetics
PubMed: 12810598 Full text @ Development
The development of vertebrate limb buds is triggered in the lateral plate mesoderm by a cascade of genes, including members of the Fgf and Wnt families, as well as the transcription factor tbx5. Fgf8, which is expressed in the intermediate mesoderm, is thought to initiate forelimb formation by activating wnt2b, which then induces the expression of tbx5 in the adjacent lateral plate mesoderm. Tbx5, in turn, is required for the activation of fgf10, which relays the limb inducing signal to the overlying ectoderm. We show that the zebrafish fgf24 gene, which belongs to the Fgf8/17/18 subfamily of Fgf ligands, acts downstream of tbx5 to activate fgf10 expression in the lateral plate mesoderm. We also show that fgf24 activity is necessary for the migration of tbx5-expressing cells to the fin bud, and for the activation of shh, but not hand2, expression in the posterior fin bud.