PUBLICATION
SHH and FGF8 play distinct roles during development of noradrenergic neurons in the locus coeruleus of the zebrafish
- Authors
- Lam, C.S., Sleptsova-Friedrich, I., Munro, A.D., and Korzh, V.
- ID
- ZDB-PUB-030602-5
- Date
- 2003
- Source
- Molecular and cellular neurosciences 22(4): 501-515 (Journal)
- Registered Authors
- Korzh, Vladimir, Lam, Eric (C.S.), Sleptsova-Freidrich, Inna
- Keywords
- none
- MeSH Terms
-
- Protein Synthesis Inhibitors/pharmacology
- Gene Expression Regulation, Developmental/genetics
- Animals
- Fibroblast Growth Factors/deficiency*
- Fibroblast Growth Factors/genetics
- Down-Regulation/genetics
- Neurons/cytology
- Neurons/metabolism*
- Mutation/genetics
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/embryology*
- Embryo, Nonmammalian/metabolism
- Norepinephrine/biosynthesis*
- Tyrosine 3-Monooxygenase/metabolism
- Fibroblast Growth Factor 8
- Activin Receptors, Type I/genetics
- Activin Receptors, Type I/metabolism
- Receptors, Growth Factor*
- Cell Survival/genetics
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism
- Bone Morphogenetic Protein Receptors, Type I
- Protein Biosynthesis/drug effects
- Protein Biosynthesis/genetics
- Trans-Activators/deficiency*
- Trans-Activators/genetics
- Up-Regulation/genetics
- Hedgehog Proteins
- Protein Serine-Threonine Kinases*
- Locus Coeruleus/cytology
- Locus Coeruleus/embryology*
- Locus Coeruleus/metabolism
- Apoptosis/genetics
- PubMed
- 12727446 Full text @ Mol. Cell Neurosci.
- CTD
- 12727446
Citation
Lam, C.S., Sleptsova-Friedrich, I., Munro, A.D., and Korzh, V. (2003) SHH and FGF8 play distinct roles during development of noradrenergic neurons in the locus coeruleus of the zebrafish. Molecular and cellular neurosciences. 22(4):501-515.
Abstract
Several signaling pathways have been implicated in the development of dopaminergic and serotonergic neurons. Here, we analyzed the formation of noradrenergic (NAergic) cells in the locus coeruleus (LC) of zebrafish. In the sonic hedgehog (shh) mutant, cells positive for tyrosine hydroxylase, a marker for putative NAergic cells in the LC were reduced. Similarly, the inhibition of translation of all hh genes and the perturbation of Shh signaling by forskolin resulted in a decrease in the number of cells. Conversely, when SHH was overexpressed, an increase in number was observed. Thus, Shh is involved in maintaining the appropriate number of cells in the LC. While elevated levels of bone morphogenetic protein 4 (BMP4) did not attenuate tyrosine hydroxylase-positive cells, exogenous fibroblast growth factor 8 (FGF8) rescued NAergic neurons in the acerebellar (ace) mutant, providing direct in vivo evidence that Fgf8 is required for the induction of NAergic neurons in the LC.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping