PUBLICATION

Zebrafish prickle, a modulator of noncanonical wnt/fz signaling, regulates gastrulation movements

Authors
Veeman, M.T., Slusarski, D.C., Kaykas, A., Louie, S.H., and Moon, R.T.
ID
ZDB-PUB-030430-2
Date
2003
Source
Current biology : CB   13(8): 680-685 (Journal)
Registered Authors
Moon, Randall T., Slusarski, Diane C.
Keywords
none
MeSH Terms
  • Adaptor Proteins, Signal Transducing
  • Animals
  • Cell Movement/genetics
  • Cell Movement/physiology*
  • DNA-Binding Proteins/genetics*
  • Drosophila Proteins/genetics*
  • Frizzled Receptors
  • Gastrula/metabolism
  • Gastrula/physiology*
  • Gene Expression
  • Glycoproteins/metabolism
  • Immunohistochemistry
  • In Situ Hybridization
  • LIM Domain Proteins
  • Phosphoproteins/metabolism
  • Proteins/metabolism
  • Signal Transduction/physiology*
  • Wnt Proteins
  • Zebrafish/embryology*
  • Zebrafish/physiology
  • Zebrafish Proteins
PubMed
12699626 Full text @ Curr. Biol.
Abstract
In addition to the canonical Wnt/beta-catenin signaling pathway, at least two noncanonical Wnt/Fz pathways have been described: the planar cell polarity (PCP) pathway in Drosophila and the Wnt/calcium pathway in vertebrate embryos. Recent work suggests that a vertebrate pathway homologous to the PCP pathway acts to regulate the convergent extension movements of gastrulation. To further test this hypothesis, we have identified two zebrafish homologs of the Drosophila PCP gene prickle (pk) [8], both of which show discrete and dynamic expression patterns during gastrulation. Both gain and loss of pk1 function cause defects in convergent extension. Pk1 localizes to both the cytoplasm and the cell membrane, and its normal localization is partially dependent on its C-terminal prenylation motif. At the cell membrane, Pk1 is frequently localized asymmetrically around the cell and can colocalize with the signaling molecule Dishevelled (Dsh). In overexpression assays, Pk1 is able to activate AP-1-mediated transcription and inhibit activation of Wnt/beta-catenin signaling. Like noncanonical Wnts, overexpression of Pk1 increases the frequency of calcium transients in zebrafish blastulae. Our results support the idea that a vertebrate PCP pathway regulates gastrulation movements and suggest that there is overlap between the PCP and Wnt/calcium pathways.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes