PUBLICATION
Cloning of transforming growth factor-beta1 (TGF-beta1) and its type II receptor from zebrafish ovary and role of TGF-beta1 in oocyte maturation
- Authors
- Kohli, G., Hu, S., Clelland, E., Di Muccio, T., Rothenstein, J., and Peng, C.
- ID
- ZDB-PUB-030425-17
- Date
- 2003
- Source
- Endocrinology 144(5): 1931-1941 (Journal)
- Registered Authors
- Peng, Chun
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence/genetics
- Animals
- Base Sequence/genetics
- Cellular Senescence/physiology
- Cloning, Molecular*
- DNA-Binding Proteins/metabolism
- Female
- Molecular Sequence Data
- Oocytes/physiology*
- Ovary/metabolism*
- Protein Serine-Threonine Kinases
- Receptors, Transforming Growth Factor beta/genetics
- Receptors, Transforming Growth Factor beta/physiology*
- Smad2 Protein
- Smad3 Protein
- Smad4 Protein
- Trans-Activators/metabolism
- Transforming Growth Factor beta/genetics
- Transforming Growth Factor beta/physiology*
- Transforming Growth Factor beta1
- Zebrafish/metabolism*
- Zebrafish Proteins*
- PubMed
- 12697700 Full text @ Endocrinology
Citation
Kohli, G., Hu, S., Clelland, E., Di Muccio, T., Rothenstein, J., and Peng, C. (2003) Cloning of transforming growth factor-beta1 (TGF-beta1) and its type II receptor from zebrafish ovary and role of TGF-beta1 in oocyte maturation. Endocrinology. 144(5):1931-1941.
Abstract
TGF-beta is a multifunctional factor involved in regulating a variety of cellular activities. In mammals, TGF-beta is known to regulate reproduction, including ovarian functions. The role of TGF-beta in lower vertebrates, such as fish, is poorly understood. To examine the role of TGF-beta in fish reproduction, cDNAs encoding TGF-beta1 and the type II TGF-beta receptor (TbetaRII) were cloned from the zebrafish ovary using PCR- based strategies. The mature peptide region of the zebrafish TGF- beta1 shows 70-85% identity with TGF-beta1 from other species. The zebrafish TbetaRII cDNA sequence is the first to be reported from a fish species, and it shows a high level of conservation at the kinase domain Using RT-PCR, we have detected mRNA expression of TGF-beta1, TbetaRII , as well as its downstream signaling molecules Smad2, 3, and 4 in ovarian follicles at different stages of development. In addition, we have examined the effect of TGF-beta1 on oocyte maturation. TGF-beta1 significantly inhibited both gonadotropin- and 17alpha, 20beta- dihydroxyprogesterone-induced oocyte maturation in a dose- and time- dependent manner. These findings demonstrate, for the first time, that TGF-beta1 plays a role in regulating oocyte maturation in fish and suggest that a TGF-beta/Smad signaling pathway is present in the zebrafish ovary.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping