PUBLICATION
Autonomous and nonautonomous functions for Hox/Pbx in branchiomotor neuron development
- Authors
- Cooper, K.L., Leisenring, W.M., and Moens, C.B.
- ID
- ZDB-PUB-030325-2
- Date
- 2003
- Source
- Developmental Biology 253(2): 200-213 (Journal)
- Registered Authors
- Cooper, Kim, Moens, Cecilia
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Cell Movement/genetics
- DNA-Binding Proteins*
- Facial Nerve/embryology
- Gene Targeting
- Genes, Homeobox*
- Glycosyltransferases/genetics
- Green Fluorescent Proteins
- Homeodomain Proteins/genetics
- Luminescent Proteins/genetics
- Mosaicism
- Motor Neurons/cytology*
- Neural Pathways/embryology
- Rhombencephalon/embryology
- Trigeminal Nerve/embryology
- Zebrafish/genetics*
- Zebrafish/growth & development*
- Zebrafish Proteins/genetics
- PubMed
- 12645925 Full text @ Dev. Biol.
Citation
Cooper, K.L., Leisenring, W.M., and Moens, C.B. (2003) Autonomous and nonautonomous functions for Hox/Pbx in branchiomotor neuron development. Developmental Biology. 253(2):200-213.
Abstract
The vertebrate branchiomotor neurons are organized in a pattern that corresponds with the segments, or rhombomeres, of the developing hindbrain and have identities and behaviors associated with their position along the anterior/posterior axis. These neurons undergo characteristic migrations in the hindbrain and project from stereotyped exit points. We show that lazarus/pbx4, which encodes an essential Hox DNA-binding partner in zebrafish, is required for facial (VIIth cranial nerve) motor neuron migration and for axon pathfinding of trigeminal (Vth cranial nerve) motor axons. We show that lzr/pbx4 is required for Hox paralog group 1 and 2 function, suggesting that Pbx interacts with these proteins. Consistent with this, lzr/pbx4 interacts genetically with hoxb1a to control facial motor neuron migration. Using genetic mosaic analysis, we show that lzr/pbx4 and hoxb1a are primarily required cell-autonomously within the facial motor neurons; however, analysis of a subtle non-cell-autonomous effect indicates that facial motor neuron migration is promoted by interactions amongst the migrating neurons. At the same time, lzr/pbx4 is required non-cell-autonomously to control the pathfinding of trigeminal motor axons. Thus, Pbx/Hox can function both cell-autonomously and non-cell-autonomously to direct different aspects of hindbrain motor neuron behavior.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping