PUBLICATION
In vivo studies of liver-type fatty acid binding protein (L-FABP) gene expression in liver of transgenic zebrafish (Danio rerio)
- Authors
- Her, G.-M., Chiang, C.-C., Chen, W.-Y., and Wu, J.-L.
- ID
- ZDB-PUB-030319-9
- Date
- 2003
- Source
- FEBS letters 538(1-3): 125-133 (Journal)
- Registered Authors
- Her, Guor Muor, Wu, Jen-Leih
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Base Sequence
- Carrier Proteins/genetics*
- Cloning, Molecular
- DNA Primers
- Fatty Acid-Binding Proteins
- Green Fluorescent Proteins
- Liver/metabolism*
- Luminescent Proteins/metabolism
- Neoplasm Proteins*
- Nerve Tissue Proteins*
- Polymerase Chain Reaction
- Zebrafish
- Zebrafish Proteins*
- PubMed
- 12633865 Full text @ FEBS Lett.
Citation
Her, G.-M., Chiang, C.-C., Chen, W.-Y., and Wu, J.-L. (2003) In vivo studies of liver-type fatty acid binding protein (L-FABP) gene expression in liver of transgenic zebrafish (Danio rerio). FEBS letters. 538(1-3):125-133.
Abstract
Mammalian liver fatty acid binding protein (L-FABP) is a small cytosolic protein in various tissues including liver, small intestine and kidney and is thought to play a crucial role in intracellular fatty acid trafficking and metabolism. To better understand its tissue-specific regulation during zebrafish hepatogenesis, we isolated 5'-flanking sequences of the zebrafish L-FABP gene and used a green fluorescent protein (GFP) transgenic strategy to generate liver-specific transgenic zebrafish. The 2.8-kb 5'-flanking sequence of zebrafish L-FABP gene was sufficient to direct GFP expression in liver primordia, first observed in 2 dpf embryos and then continuously to the adult stage. This pattern of transgenic expression is consistent with the expression pattern of the endogenous gene. F2 inheritance rates of 42-51% in all the seven transgenic lines were consistent with the ratio of Mendelian segregation. Further, hhex and zXbp-1 morphants displayed a visible liver defect, which suggests that it is possible to establish an in vivo system for screening genes required for liver development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping