PUBLICATION

Gene duplication and spectral diversification of cone visual pigments of zebrafish

Authors
Chinen, A., Hamaoka, T., Yamada, Y., and Kawamura, S.
ID
ZDB-PUB-030307-9
Date
2003
Source
Genetics   163(2): 663-675 (Journal)
Registered Authors
Kawamura, Shoji
Keywords
none
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Blotting, Southern
  • Gene Duplication*
  • Molecular Sequence Data
  • Phylogeny
  • Physical Chromosome Mapping
  • Rod Opsins/chemistry
  • Rod Opsins/genetics*
  • Sequence Alignment
  • Spectrophotometry
  • Zebrafish/genetics*
  • Zebrafish/metabolism
(all 13)
PubMed
12618404 Full text @ Genetics
Abstract
Zebrafish is becoming a powerful animal model for the study of vision but the genomic organization and variation of its visual opsins have not been fully characterized. We show here that zebrafish has two red (LWS-1 and LWS-2), four green (RH2-1, RH2-2, RH2-3, and RH2-4), and single blue (SWS2) and ultraviolet (SWS1) opsin genes in the genome, among which LWS-2, RH2-2, and RH2-3 are novel. SWS2, LWS-1, and LWS-2 are located in tandem and RH2-1, RH2-2, RH2-3, and RH2-4 form another tandem gene cluster. The peak absorption spectra (lambdamax) of the reconstituted photopigments from the opsin cDNAs differed markedly among them: 558 nm (LWS-1), 548 nm (LWS-2), 467 nm (RH2-1), 476 nm (RH2-2), 488 nm (RH2-3), 505 nm (RH2-4), 355 nm (SWS1), 416 nm (SWS2), and 501 nm (RH1, rod opsin). The quantitative RT-PCR revealed a considerable difference among the opsin genes in the expression level in the retina. The expression of the two red opsin genes and of three green opsin genes, RH2-1, RH2-3, and RH2-4, is significantly lower than that of RH2-2, SWS1, and SWS2. These findings must contribute to our comprehensive understanding of visual capabilities of zebrafish and the evolution of the fish visual system and should become a basis of further studies on expression and developmental regulation of the opsin genes.
Genes / Markers
Marker Marker Type Name
cxxc1bGENECXXC finger protein 1b
opn1lw1GENEopsin 1 (cone pigments), long-wave-sensitive, 1
opn1lw2GENEopsin 1 (cone pigments), long-wave-sensitive, 2
opn1mw1GENEopsin 1 (cone pigments), medium-wave-sensitive, 1
opn1mw2GENEopsin 1 (cone pigments), medium-wave-sensitive, 2
opn1mw3GENEopsin 1 (cone pigments), medium-wave-sensitive, 3
opn1mw4GENEopsin 1 (cone pigments), medium-wave-sensitive, 4
opn1sw1GENEopsin 1 (cone pigments), short-wave-sensitive 1
opn1sw2GENEopsin 1 (cone pigments), short-wave-sensitive 2
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Figures
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Expression
Phenotype
No data available
Mutations / Transgenics
No data available
Human Disease / Model
No data available
Sequence Targeting Reagents
No data available
Fish
No data available
Antibodies
No data available
Orthology
Engineered Foreign Genes
No data available
Mapping
Entity Type Entity Symbol Location
GENEcxxc1bChr: 11 Details
GENEopn1lw1Chr: 11 Details
GENEopn1mw1Chr: 6 Details
GENEopn1mw2Chr: 6 Details
GENEopn1mw3Chr: 6 Details
GENEopn1mw4Chr: 6 Details
GENEopn1sw2Chr: 11 Details
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