PUBLICATION

The role of a retinoic acid response element in establishing the anterior neural expression border of Hoxd4 transgenes

Authors
Nolte, C., Amores, A., Nagy Kovacs, E., Postlethwait, J., and Featherstone, M.
ID
ZDB-PUB-030304-11
Date
2003
Source
Mechanisms of Development   120(3): 325-335 (Journal)
Registered Authors
Amores, Angel, Postlethwait, John H.
Keywords
none
MeSH Terms
  • Retinoid X Receptors
  • Animals
  • Enhancer Elements, Genetic
  • Conserved Sequence
  • Body Patterning/genetics*
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
  • Tretinoin/metabolism*
  • Mice, Transgenic
  • Dimerization
  • Transcription Factors/genetics*
  • Transcription Factors/metabolism
  • Mice
  • Mice, Inbred Strains
  • Base Sequence
  • Response Elements*
  • Half-Life
  • Nervous System/embryology*
  • Transgenes
  • beta-Galactosidase/genetics
  • beta-Galactosidase/metabolism
  • Promoter Regions, Genetic
  • Gene Expression Regulation, Developmental
  • Molecular Sequence Data
  • Mutation
  • Homeodomain Proteins*
  • Receptors, Retinoic Acid/genetics
  • Receptors, Retinoic Acid/metabolism
(all 28)
PubMed
12591602 Full text @ Mech. Dev.
Abstract
The zebrafish hoxd4a locus was compared to its murine ortholog, Hoxd4. The sequence of regulatory elements, including a DR5 type retinoic acid response element (RARE) required for Hoxd4 neural enhancer activity, are highly conserved. Additionally, zebrafish and mouse neural enhancers function identically in transgenic mouse embryos. We tested whether sequence conservation reflects functional importance by altering the spacing and sequence of the RARE in the Hoxd4 neural enhancer. Stabilizing receptor-DNA interactions did not anteriorize transgene expression. By contrast, conversion of the RARE from a DR5 to a DR2 type element decreased receptor-DNA stability and posteriorized expression. Hence, the setting of the Hox anterior expression border is not a simple function of the affinity of retinoid receptors for their cognate element.
Genes / Markers
Figures
No images available
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
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Citation
Nolte, C., Amores, A., Nagy Kovacs, E., Postlethwait, J., and Featherstone, M. (2003) The role of a retinoic acid response element in establishing the anterior neural expression border of Hoxd4 transgenes. Mechanisms of Development. 120(3):325-335.