PUBLICATION
cDNA sequences of the authentic keratins 8 and 18 in zebrafish
- Authors
- Schaffeld, M., Knappe, M., Hunzinger, C., and Markl, J.
- ID
- ZDB-PUB-030211-5
- Date
- 2003
- Source
- Differentiation; research in biological diversity 71(1): 73-82 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Keratins/chemistry*
- Keratins/genetics
- Keratins/metabolism
- Humans
- Tissue Distribution
- Zebrafish Proteins/chemistry*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Zebrafish
- Phylogeny
- Sequence Alignment
- Molecular Sequence Data
- Keratin-8
- Keratin-18
- Amino Acid Sequence
- Cloning, Molecular
- Animals
- PubMed
- 12558605 Full text @ Differentiation
Citation
Schaffeld, M., Knappe, M., Hunzinger, C., and Markl, J. (2003) cDNA sequences of the authentic keratins 8 and 18 in zebrafish. Differentiation; research in biological diversity. 71(1):73-82.
Abstract
From the zebrafish Danio rerio, we have cDNA cloned and sequenced a novel type II and a novel type I keratin, termed DreK8 and DreK18, respectively. We identified DreK8/18 as the true orthologs of the human keratin pair K8/18 as follows: (i) MALDI-MS assignment to the biochemically identified K8 and K18 candidates that are co-expressed in simple epithelia and absent in epidermal keratinocytes; (ii) multiple sequence alignments and phylogenetic tree analysis, showing that DreK8, within the phylogenetic tree of type II keratins, forms a highly bootstrap-supported branch together with K8 from goldfish and rainbow trout, whereas DreK18, within the phylogenetic tree of type I keratins, groups with the K18 sequences from all other vertebrates studied; (iii) presence of a conserved motif in the tail domain of DreK8 ( VxKxxETxDGxxVSESSxV) that is typical for all hitherto sequenced K8 orthologs. Moreover, several zebrafish type II keratin sequences published by other authors have now been assigned to epidermal keratins , previously identified biochemically.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping