PUBLICATION
Translocation of CaM kinase II to synaptic sites in vivo
- Authors
- Gleason, M.R., Higashijima, S.I., Dallman, J., Liu, K., Mandel, G., and Fetcho, J.R.
- ID
- ZDB-PUB-030211-14
- Date
- 2003
- Source
- Nature Neuroscience 6(3): 217-218 (Journal)
- Registered Authors
- Fetcho, Joseph R., Higashijima, Shin-ichi, Liu, Katharine S.
- Keywords
- none
- MeSH Terms
-
- Neuronal Plasticity/physiology
- Interneurons/drug effects
- Interneurons/metabolism
- Electric Stimulation
- Promoter Regions, Genetic
- Bacterial Proteins/genetics
- Synaptic Transmission/physiology
- Calcium-Calmodulin-Dependent Protein Kinase Type 2
- Synapses/metabolism*
- ELAV Proteins
- Green Fluorescent Proteins
- Zebrafish Proteins*
- Nerve Tissue Proteins/genetics
- Recombinant Fusion Proteins/genetics
- Recombinant Fusion Proteins/metabolism
- Zebrafish
- Calcium-Calmodulin-Dependent Protein Kinases/metabolism*
- Animals
- RNA-Binding Proteins/genetics
- Microinjections
- Video Recording
- ELAV-Like Protein 3
- Receptors, N-Methyl-D-Aspartate/metabolism
- Glycine/pharmacology
- Luminescent Proteins/genetics
- Protein Transport/drug effects
- Protein Transport/physiology
- Glutamic Acid/pharmacology
- Fluorescent Dyes
- Skin/innervation
- PubMed
- 12563265 Full text @ Nat. Neurosci.
Citation
Gleason, M.R., Higashijima, S.I., Dallman, J., Liu, K., Mandel, G., and Fetcho, J.R. (2003) Translocation of CaM kinase II to synaptic sites in vivo. Nature Neuroscience. 6(3):217-218.
Abstract
The idea that calcium/calmodulin-dependent protein kinase II (CaMKII) is strategically localized to excitatory synapses to exert its important role in long-term potentiation and other forms of neuronal plasticity is supported by the binding of CaMKII to isolated postsynaptic densities (PSD) in biochemical assays and by the finding in cultured neurons that PSD clusters of green fluorescent protein (GFP)-tagged CaMKII form in response to glutamate application or direct electrical stimulation. The observation that CaMKII also forms large clusters in response to ischemic insults, however, questions the physiological relevance of such translocations. Here we show that in intact zebrafish, repeated sensory stimulation resulted in reproducible and reversible translocation of GFP-CaMKII to the PSD in an identified interneuron in a sensorimotor circuit.
Genes / Markers
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Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
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