PUBLICATION
Membrane properties related to the firing behavior of zebrafish motoneurons
- Authors
- Buss, R.R., Bourque, C.W., and Drapeau, P.
- ID
- ZDB-PUB-030211-11
- Date
- 2003
- Source
- Journal of neurophysiology 89(2): 657-664 (Journal)
- Registered Authors
- Buss, Robert, Drapeau, Pierre
- Keywords
- none
- MeSH Terms
-
- 4-Aminopyridine/analogs & derivatives*
- 4-Aminopyridine/pharmacology
- Action Potentials/drug effects
- Action Potentials/physiology*
- Anesthetics, Local/pharmacology
- Animals
- Calcium Channels/physiology
- Cobalt/pharmacology
- Motor Neurons/physiology*
- Patch-Clamp Techniques
- Potassium Channel Blockers/pharmacology
- Potassium Channels/physiology
- Sodium Channels/physiology
- Swimming/physiology
- Tetraethylammonium/pharmacology
- Tetrodotoxin/pharmacology
- Zebrafish
- PubMed
- 12574443 Full text @ J. Neurophysiol.
Citation
Buss, R.R., Bourque, C.W., and Drapeau, P. (2003) Membrane properties related to the firing behavior of zebrafish motoneurons. Journal of neurophysiology. 89(2):657-664.
Abstract
The physiological and pharmacological properties of the motoneuron membrane and action potential were investigated in larval zebrafish using whole cell patch current-clamp recording techniques. Action potentials were eliminated in tetrodotoxin, repolarized by tetraethylammonium (TEA) and 3,4-diaminopyridine (3,4-AP)-sensitive potassium conductances, and had a cobalt-sensitive, high-threshold calcium component. Depolarizing current injection evoked a brief (approximately 10-30 ms) burst of action potentials that was terminated by strong, outwardly rectifying voltage-activated potassium and calcium-dependent conductances. In the presence of intracellular cesium ions, a prolonged plateau potential often followed brief depolarizations. During larval development (hatching to free-swimming), the resting membrane conductance increased in a population of motoneurons, which tended to reduce the apparent outward rectification of the membrane. The conductances contributing to action potential burst termination are hypothesized to play a role in patterning the synaptically driven motoneuron output in these rapidly swimming fish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping