PUBLICATION
Involvement of cyclic adenosine 3',5'-monophosphate in the differential regulation of activin betaA and betaB expression by gonadotropin in the zebrafish ovarian follicle cells
- Authors
- Wang, Y. and Ge, W.
- ID
- ZDB-PUB-030210-7
- Date
- 2003
- Source
- Endocrinology 144(2): 491-9 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Cells, Cultured
- Gene Expression/drug effects
- Gene Expression/physiology
- Bucladesine/pharmacology
- Phosphodiesterase Inhibitors/pharmacology
- Cyclic AMP/metabolism*
- Amino Acid Sequence
- Colforsin/pharmacology
- DNA, Complementary
- Inhibin-beta Subunits/genetics*
- Gonadotropins/pharmacology*
- Cyclic AMP-Dependent Protein Kinases/metabolism
- 1-Methyl-3-isobutylxanthine/pharmacology
- Zebrafish
- Reverse Transcriptase Polymerase Chain Reaction/standards
- Female
- Humans
- Molecular Sequence Data
- Reproducibility of Results
- Cloning, Molecular
- Ovarian Follicle/cytology
- Ovarian Follicle/physiology*
- Animals
- PubMed
- 12538609 Full text @ Endocrinology
Citation
Wang, Y. and Ge, W. (2003) Involvement of cyclic adenosine 3',5'-monophosphate in the differential regulation of activin betaA and betaB expression by gonadotropin in the zebrafish ovarian follicle cells. Endocrinology. 144(2):491-9.
Abstract
Activin is a dimeric protein consisting of two similar but distinct beta -subunits, betaA and betaB. In our previous studies, both activin A ( betaAbetaA) and activin B (betaBbetaB) have been demonstrated to stimulate oocyte maturation and promote oocyte maturational competence in the zebrafish. Follistatin, a specific activin-binding protein, can block both activin- and gonadotropin-induced final oocyte maturation in vitro, suggesting that activin is likely a downstream mediator of gonadotropin actions in the zebrafish ovary. In the present study, a full-length cDNA encoding zebrafish ovarian activin betaA was cloned and sequenced. The precursor of zebrafish activin betaA consists of 395 amino acids and its mature region exhibits about 78% homology with that of mammals. Using an in vitro primary culture of the ovarian follicle cells and semiquantitative RT-PCR assays, we examined the regulation of activin betaA and betaB expression by human chorionic gonadotropin (hCG ) and its intracellular signal transduction mechanisms. hCG (15 IU/ml) increased the mRNA level of activin betaA-subunit; however, it significantly down-regulated the steady-state expression level of activin betaB in a time- and dose-dependent manner. The differential regulation of the two beta-subunits by hCG could be mimicked by 3- isobutyl-1-methylxanthine, forskolin, and dibutyryl-cAMP, suggesting involvement of the intracellular cAMP pathway. Interestingly, H89 (a specific inhibitor of protein kinase A, PKA) could effectively block hCG - and forskolin-stimulated activin betaA expression at 10 micro M, but it was unable to reverse the inhibitory effects of hCG and forskolin on betaB expression. This suggests that the hCG-stimulated activin betaA expression is dependent on the activation of the cAMP-PKA pathway, whereas the inhibitory effect of hCG on activin betaB expression is likely mediated by PKA-independent pathway(s).
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping