PUBLICATION
Variations in DNA (cytosine-5)-methyltransferase-1 expression during oogenesis and early development of the zebrafish
- Authors
- Mhanni, A.A. and McGowan, R.A.
- ID
- ZDB-PUB-030117-5
- Date
- 2002
- Source
- Development genes and evolution 212(11): 530-533 (Journal)
- Registered Authors
- McGowan, Ross
- Keywords
- DNA cytosine-5-methyltransferase - DNA MTase - dnmt1 - Zebrafish - Methylation
- MeSH Terms
-
- Animals
- DNA (Cytosine-5-)-Methyltransferases/genetics*
- Embryo, Nonmammalian/enzymology
- Female
- Gene Expression Regulation, Developmental*
- Gene Expression Regulation, Enzymologic
- Molecular Sequence Data
- Oogenesis/genetics*
- Ovary/enzymology
- Transcriptional Activation
- Zebrafish/embryology*
- PubMed
- 12459921 Full text @ Dev. Genes Evol.
Citation
Mhanni, A.A. and McGowan, R.A. (2002) Variations in DNA (cytosine-5)-methyltransferase-1 expression during oogenesis and early development of the zebrafish. Development genes and evolution. 212(11):530-533.
Abstract
We report the determination of zebrafish DNA (cytosine-5-) methyltransferase ( dnmt-1) temporal and spatial patterns of expression in gonadal tissues and during early development. Only one dnmt-1 message of around 5 kb was observed in all tissues examined and its levels were highest in gonadal tissues. During the course of oogenesis, early oocytes contain significant amounts of dnmt-1 transcript while message abundance declines as oocytes mature. During early embryogenesis message levels remain low until the blastula stage. Methyltransferase enzyme assays reveal that the maternal dnmt-1 message accumulated during oogenesis is translated into protein presumably providing necessary dnmt-1 stockpiles to support early embryonic development prior to zygotic gene activation. Such spatial and temporal regulation of dnmt-1 expression suggests specific functions for the enzyme during oogenesis and early development of zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping