PUBLICATION
Developmental changes in the expression of growth hormone-releasing hormone and pituitary adenylate cyclase-activating polypeptide in zebrafish
- Authors
- Krueckl, S.L., Fradinger, E.A., and Sherwood, N.M.
- ID
- ZDB-PUB-030115-33
- Date
- 2003
- Source
- The Journal of comparative neurology 455(3): 396-405 (Journal)
- Registered Authors
- Fradinger, Erica, Krueckl, Sandra, Sherwood, Nancy M.
- Keywords
- neuropeptides, development, mRNA, gene expression, alternative splicing, in situ hybridization
- MeSH Terms
-
- Animals
- Base Sequence
- Blastula/metabolism
- Brain/embryology
- Brain/growth & development*
- Gastrula/metabolism
- Growth Hormone-Releasing Hormone/chemistry
- Growth Hormone-Releasing Hormone/isolation & purification
- Growth Hormone-Releasing Hormone/metabolism*
- In Situ Hybridization
- Molecular Sequence Data
- Neuropeptides/chemistry
- Neuropeptides/isolation & purification
- Neuropeptides/metabolism*
- Pituitary Adenylate Cyclase-Activating Polypeptide
- Receptors, Vasoactive Intestinal Peptide
- Reverse Transcriptase Polymerase Chain Reaction
- Zebrafish/embryology*
- Zebrafish/growth & development
- Zebrafish Proteins
- PubMed
- 12483690 Full text @ J. Comp. Neurol.
Citation
Krueckl, S.L., Fradinger, E.A., and Sherwood, N.M. (2003) Developmental changes in the expression of growth hormone-releasing hormone and pituitary adenylate cyclase-activating polypeptide in zebrafish. The Journal of comparative neurology. 455(3):396-405.
Abstract
Growth hormone-releasing hormone (GHRH) and pituitary adenylate cyclase-activating polypeptide (PACAP) are structurally and functionally related members of the glucagon superfamily, a group of hormones important in development, growth, and metabolism. Our objectives were to determine the developmental expression pattern of the ghrh-pacap1 gene using the zebrafish model. The temporal and spatial expression pattern of the ghrh-pacap1 gene was examined by RT-PCR and in situ hybridization. In zebrafish, the ghrh-pacap1 mRNA transcript was expressed throughout development beginning at the transition between the blastula and gastrula periods. During midgastrulation, alternative splicing resulted in the generation of a novel transcript lacking the cryptic peptide. During the segmentation period, expression was localized to the neural tube, developing eye, and neural crest; strong expression was found in the developing cerebellum. Later in development, expression was localized in the hatching gland and developing pharyngeal arches. The temporal and spatial expression pattern of the ghrh-pacap1 transcript suggests that these hormones may modulate patterning during development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping