Analysis of gene function in the zebrafish retina
- Malicki, J., Jo, H., Wei, X., Hsiung, M., and Pujic, Z.
- Methods (San Diego, Calif.) 28(4): 427-438 (Journal)
- Registered Authors
- Jo, Hakryul, Malicki, Jarema, Pujic, Zac, Wei, Xiangyun
- Zebrafish; retina; Morpholino; Overexpression; Knockdown; Eye; Development; Neuronal; Heat shock; Transgenic
- MeSH Terms
- Animals, Genetically Modified
- Base Sequence
- Gene Expression Regulation, Developmental/genetics*
- Molecular Biology/methods*
- Molecular Sequence Data
- Retina/growth & development*
- 12507461 Full text @ Methods
Malicki, J., Jo, H., Wei, X., Hsiung, M., and Pujic, Z. (2002) Analysis of gene function in the zebrafish retina. Methods (San Diego, Calif.). 28(4):427-438.
Mutagenesis screens in zebrafish have uncovered several hundred mutant alleles affecting the development of the retina and established the zebrafish as one of the leading models of vertebrate eye development. In addition to forward genetic mutagenesis approaches, gene function in the zebrafish embryo is being studied using several reverse genetic techniques. Some of these rely on the overexpression of a gene product, others take advantage of antisense oligonucleotides to block function of selected loci. Here we describe these methods in the context of the developing eye.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes