PUBLICATION
Ccd1, a novel protein with a DIX domain, is a positive regulator in the Wnt signaling during zebrafish neural patterning
- Authors
- Shiomi, K., Uchida, H., Keino-Masu, K., and Masu, M.
- ID
- ZDB-PUB-030114-1
- Date
- 2003
- Source
- Current biology : CB 13(1): 73-77 (Journal)
- Registered Authors
- Shiomi, Kensuke
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Axin Protein
- Binding Sites
- Body Patterning/physiology*
- Cytoskeletal Proteins
- DNA-Binding Proteins/metabolism
- Embryo, Nonmammalian
- Eye Abnormalities/genetics
- Eye Proteins
- Gene Expression Regulation, Developmental
- Homeodomain Proteins/genetics
- Lymphoid Enhancer-Binding Factor 1
- Molecular Sequence Data
- Nervous System/embryology*
- Nervous System/metabolism
- Paired Box Transcription Factors
- Protein Serine-Threonine Kinases/genetics
- Protein Structure, Tertiary
- Proteins/genetics
- Proteins/metabolism*
- Proto-Oncogene Proteins c-myc/genetics
- Proto-Oncogene Proteins c-myc/metabolism
- Repressor Proteins*
- Sequence Homology, Amino Acid
- Signal Transduction*
- Transcription Factors/metabolism
- Transfection
- Wnt Proteins
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism*
- PubMed
- 12526749 Full text @ Curr. Biol.
Citation
Shiomi, K., Uchida, H., Keino-Masu, K., and Masu, M. (2003) Ccd1, a novel protein with a DIX domain, is a positive regulator in the Wnt signaling during zebrafish neural patterning. Current biology : CB. 13(1):73-77.
Abstract
Wnt signaling plays a crucial role in directing cell differentiation, polarity, and growth. In the canonical pathway, Wnt receptors activate Dishevelled (Dvl), which then blocks the degradation of a key signal transducer, beta-catenin, leading to the nuclear accumulation of beta-catenin and induction of Wnt target genes through TCF/LEF family transcription factors. Here we identified a novel zebrafish gene encoding Ccd1, which possesses a DIX (Dishevelled-Axin) domain. DIX domains are essential for the signal transduction of two major Wnt downstream mediators, Dvl and Axin. Ccd1 formed homomeric and heteromeric complexes with Dvl and Axin and activated TCF-dependent transcription in vitro. In addition, overexpression of ccd1 in zebrafish embryos led to a reduction in the size of the eyes and forebrain (posteriorization), as seen with wnt8 overexpression, whereas a dominant-negative ccd1 (DN-ccd1) caused the opposite phenotype. Furthermore, the Wnt activation phenotype induced by ccd1 was inhibited by the expression of axin1 or DN-ccd1, and the wnt8 overexpression phenotype was rescued by DN-ccd1, suggesting that Ccd1 functions downstream of the Wnt receptor and upstream of Axin. These results indicate that Ccd1 is a novel positive regulator in this Wnt signaling pathway during zebrafish neural patterning.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping