PUBLICATION

Effects of maternal exposure to estrogen and PCB on different life stages of zebrafish Danio rerio

Authors
Olsson, P.-E., Westerlund, L., Teh, S.J., Billsson, K., Berg, A.H., Tysklind, M., Nilsson, J., Eriksson, L.-O., and Hinton, D.E.
ID
ZDB-PUB-030106-8
Date
1999
Source
Ambio   28(1): 100-106 (Journal)
Registered Authors
Keywords
PCB; Reproduction; Biological development; Hormones; Fecundity; Mortality causes; Organism morphology; Histology; Fish larvae; Gonads; Pollution effects; Danio rerio
MeSH Terms
none
PubMed
none
Abstract
PCBs have been found to impair both reproduction and development in fish. We have investigated the effects of 3 PCB congeners and the estrogenic hormone 17 beta -estradiol on fecundity, early life-stage mortality, gross morphology and histology of zebrafish (Danio rerio). While none of the studied substances reduced fecundity, they increased embryo and larval mortality. The most severe effects on viability were observed following treatment with 17 beta -estradiol or the weakly estrogenic PCB-104. Following 17 beta -estradiol or PCB-104 exposure, mortality continued through the yolksac absorption phase. PCB-60, on the other hand, resulted in mortality between the 30% epiboly stage and 75% epiboly stage. At the same time as embryos started to die, embryo development and hatching were delayed. PCB-1 90 showed only moderate effects on early-life stage mortality. The fish were reared until sexual maturation whereafter they were subjected to gross morphological and histological analyses. While all studied substances resulted in effects on offspring, the observation that estrogenic substances are highly embryotoxic, raises concern that endocrine disrupting substances may severely reduce fish populations in polluted areas.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping