PUBLICATION
Mps1 defines a proximal blastemal proliferative compartment essential for zebrafish fin regeneration
- Authors
- Poss, K.D., Nechiporuk, A., Hillam, A.M., Johnson, S.L., and Keating, M.T.
- ID
- ZDB-PUB-030102-3
- Date
- 2002
- Source
- Development (Cambridge, England) 129(22): 5141-5149 (Journal)
- Registered Authors
- Johnson, Stephen L., Keating, Mark T., Nechiporuk, Alex, Poss, Kenneth D.
- Keywords
- zebrafish; fin; regeneration; blastema; mps1
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Body Patterning
- Cell Division/genetics
- Ethylnitrosourea/toxicity
- Extremities/embryology
- Extremities/physiology
- Female
- Genetic Techniques
- Limb Buds
- Male
- Molecular Sequence Data
- Mutation
- Protein Serine-Threonine Kinases/drug effects
- Protein Serine-Threonine Kinases/genetics*
- Protein Serine-Threonine Kinases/metabolism*
- Protein-Tyrosine Kinases/drug effects
- Protein-Tyrosine Kinases/genetics*
- Protein-Tyrosine Kinases/metabolism*
- Regeneration*
- Zebrafish/physiology*
- Zebrafish Proteins/drug effects
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism*
- PubMed
- 12399306 Full text @ Development
Citation
Poss, K.D., Nechiporuk, A., Hillam, A.M., Johnson, S.L., and Keating, M.T. (2002) Mps1 defines a proximal blastemal proliferative compartment essential for zebrafish fin regeneration. Development (Cambridge, England). 129(22):5141-5149.
Abstract
One possible reason why regeneration remains enigmatic is that the dominant organisms used for studying regeneration are not amenable to genetic approaches. We mutagenized zebrafish and screened for temperature-sensitive defects in adult fin regeneration. The nightcap mutant showed a defect in fin regeneration that was first apparent at the onset of regenerative outgrowth. Positional cloning revealed that nightcap encodes the zebrafish orthologue of mps1, a kinase required for the mitotic checkpoint. mps1 expression was specifically induced in the proximal regeneration blastema, a group of cells that normally proliferate intensely during outgrowth. The nightcap mutation caused severe defects in these cells. However, msxb-expressing blastemal cells immediately distal to this proliferative region did not induce mps1 and were retained in mutants. These results indicate that the proximal blastema comprises an essential subpopulation of the fin regenerate defined by the induction and function of Mps1. Furthermore, we show that molecular mechanisms of complex tissue regeneration can now be dissected using zebrafish genetics.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping