PUBLICATION
Embryonic retinal gene expression in sonic-you mutant zebrafish
- Authors
- Stenkamp, D.L., Frey, R.A., Mallory, D.E., and Shupe, E.E.
- ID
- ZDB-PUB-021106-7
- Date
- 2002
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 225(3): 344-350 (Journal)
- Registered Authors
- Frey, Ruth, Stenkamp, Deborah L.
- Keywords
- sonic hedgehog; tiggy-winkle hedgehog; crx; rx; neuroD; pax6; ath5; rod opsin; red cone opsin; photoreceptor; retina; zebrafish
- MeSH Terms
-
- Animals
- Cell Differentiation/physiology
- Cell Division/physiology
- Gene Expression Regulation, Developmental*
- Hedgehog Proteins
- Mutation/physiology
- Phenotype
- Retina/cytology
- Retina/embryology*
- Retina/physiology
- Trans-Activators/genetics*
- Zebrafish
- PubMed
- 12412019 Full text @ Dev. Dyn.
Citation
Stenkamp, D.L., Frey, R.A., Mallory, D.E., and Shupe, E.E. (2002) Embryonic retinal gene expression in sonic-you mutant zebrafish. Developmental Dynamics : an official publication of the American Association of Anatomists. 225(3):344-350.
Abstract
Hedgehog (Hh) signaling is required for proper eye development in vertebrates; known roles for Hh in the zebrafish include regulation of eye morphogenesis, ganglion cell neurogenesis, and photoreceptor differentiation. To gain insight into the mechanisms by which Hh signaling influences these developmental events, we have examined proliferation, cell death, and expression patterns of several retinal genes in the eyes of embryonic zebrafish lacking the sonic hedgehog gene We find that features of the eye phenotype of the sonic-you (syu) mutant are consistent with multiple roles for the Hh signal during retinal development. Most interestingly, half of the mutant retinas failed to initiate cell differentiation and, instead, retained a neuroepithelial appearance. In the other half of the mutants, retinal cell differentiation was initiated, but not fully propagated. We also find that Hh signaling is important for retinal cell proliferation and retinal cell survival; together, these functions provide an explanation for progressive microphthalmia in the syu-/- mutant.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping