Inhibitory interactions controlling organizer activity in fish
- Peyriéras, N., Lu, Y., Renucci, A., Lemarchandel, V., and Rosa, F.
- Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie 319(12): 1107-1112 (Journal)
- Registered Authors
- Lemarchandel, Valerie, Renucci, Armand, Rosa, Frederic
- MeSH Terms
- Mesoderm/drug effects
- Nucleolus Organizer Region*
- RNA, Messenger/genetics
- RNA, Messenger/pharmacology
- Transforming Growth Factor beta/genetics
Peyriéras, N., Lu, Y., Renucci, A., Lemarchandel, V., and Rosa, F. (1996) Inhibitory interactions controlling organizer activity in fish. Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie. 319(12):1107-1112.
An experimental system allowing the observation of 2 active organizing centers during zebrafish development is described. It was achieved by injection into a single marginal cell at the 16-cell stage of TARAM-A-D mRNA. TARAM-A-D was previously described as the mutated constitutive form of a type I receptor for transforming growth factor beta. In 80% of the injected embryos, 2 distinct organizers were observed at the onset of gastrulation. At the end of gastrulation, these embryos showed duplicated axial structures. Nevertheless, only 25% of the injected embryos displayed a recognizable axis duplication after 1 day of development. This paradox is taken as an evidence for suppressive effects exerted in a reciprocal manner when more than 1 organizing center is present.
Genes / Markers
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Engineered Foreign Genes
Errata and Notes