ZFIN ID: ZDB-PUB-021105-6
Visualization of rod photoreceptor development using GFP-transgenic zebrafish
Hamaoka, T., Takechi, M., Chinen, A., Nishiwaki, Y., and Kawamura, S.
Date: 2002
Source: Genesis (New York, N.Y. : 2000)   34(3): 215-220 (Journal)
Registered Authors: Kawamura, Shoji, Nishiwaki, Yuko
Keywords: rod; opsin; GFP; zebrafish; retina
MeSH Terms:
  • 5' Untranslated Regions/genetics
  • Animals
  • Animals, Genetically Modified
  • Fluorescent Antibody Technique
  • Green Fluorescent Proteins
  • Luminescent Proteins/genetics*
  • Luminescent Proteins/metabolism*
  • Microscopy, Fluorescence
  • Retina/cytology
  • Retinal Rod Photoreceptor Cells/embryology*
  • Rod Opsins/genetics
  • Staining and Labeling/methods*
  • Zebrafish/embryology*
  • Zebrafish/genetics*
PubMed: 12395387 Full text @ Genesis
ABSTRACT
Summary: Zebrafish retina contains five morphologically distinct classes of photoreceptors, each expressing a distinct type of opsin gene. Molecular mechanisms underlying specification of opsin expression and differentiation among the cell types are largely unknown. This is partly because mutants affected with expression of a particular class of opsin gene are difficult to find. In this study we established the transgenic lines of zebrafish carrying green fluorescent protein (GFP) gene under the 1.1-kb and 3.7-kb upstream regions of the rod-opsin gene. In transgenic fish, GFP expression initiated and proceeded in the same spatiotemporal pattern with rod-opsin gene. The retinal section from adult transgenic fish showed GFP expression throughout the rod cell layer. These results indicate that the proximal 1.1-kb region is sufficient to drive gene expression in all rod photoreceptor cells. These transgenic fish should facilitate screening of mutants affected specifically with rod-opsin expression or rod cell development by visualization of rod cells by GFP. genesis 34:215-220, 2002.
ADDITIONAL INFORMATION