PUBLICATION
Visualization of rod photoreceptor development using GFP-transgenic zebrafish
- Authors
- Hamaoka, T., Takechi, M., Chinen, A., Nishiwaki, Y., and Kawamura, S.
- ID
- ZDB-PUB-021105-6
- Date
- 2002
- Source
- Genesis (New York, N.Y. : 2000) 34(3): 215-220 (Journal)
- Registered Authors
- Kawamura, Shoji, Nishiwaki, Yuko
- Keywords
- rod; opsin; GFP; zebrafish; retina
- MeSH Terms
-
- 5' Untranslated Regions/genetics
- Animals
- Animals, Genetically Modified
- Fluorescent Antibody Technique
- Green Fluorescent Proteins
- Luminescent Proteins/genetics*
- Luminescent Proteins/metabolism*
- Microscopy, Fluorescence
- Retina/cytology
- Retinal Rod Photoreceptor Cells/embryology*
- Rod Opsins/genetics
- Staining and Labeling/methods*
- Zebrafish/embryology*
- Zebrafish/genetics*
- PubMed
- 12395387 Full text @ Genesis
Citation
Hamaoka, T., Takechi, M., Chinen, A., Nishiwaki, Y., and Kawamura, S. (2002) Visualization of rod photoreceptor development using GFP-transgenic zebrafish. Genesis (New York, N.Y. : 2000). 34(3):215-220.
Abstract
Summary: Zebrafish retina contains five morphologically distinct classes of photoreceptors, each expressing a distinct type of opsin gene. Molecular mechanisms underlying specification of opsin expression and differentiation among the cell types are largely unknown. This is partly because mutants affected with expression of a particular class of opsin gene are difficult to find. In this study we established the transgenic lines of zebrafish carrying green fluorescent protein (GFP) gene under the 1.1-kb and 3.7-kb upstream regions of the rod-opsin gene. In transgenic fish, GFP expression initiated and proceeded in the same spatiotemporal pattern with rod-opsin gene. The retinal section from adult transgenic fish showed GFP expression throughout the rod cell layer. These results indicate that the proximal 1.1-kb region is sufficient to drive gene expression in all rod photoreceptor cells. These transgenic fish should facilitate screening of mutants affected specifically with rod-opsin expression or rod cell development by visualization of rod cells by GFP. genesis 34:215-220, 2002.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping