PUBLICATION

Artificial chromosome transgenesis reveals long-distance negative regulation of rag1 in zebrafish

Authors
Jessen, J.R.
ID
ZDB-PUB-021016-60
Date
1999
Source
Ph.D. Thesis : (Thesis)
Registered Authors
Jessen, Jason R.
Keywords
none
MeSH Terms
none
PubMed
none
Abstract
Despite the essential roles played by the recombination activating genes (rag1 and rag2 ) during V(D)J recombination, the mechanisms that restrict their expression to lymphoid cells are undefined. Using a novel approach to achieve artificial chromosome transgenesis in zebrafish, we demonstrate that distal regulatory elements are critical to suppress rag1 expression in inappropriate tissues. In contrast to smaller reporter gene constructs, 125 and 75 kb artificial chromosomes containing the zebrafish rag genomic locus directed GFP expression in a pattern reflective of endogenous rag1. Mapping experiments identified a positive element 5? of rag1 that enhances GFP expression in both lymphoid and non-lymphoid tissues and a negative element 5? of rag1 that specifically suppresses GFP expression in the skeletal muscle. Our transgenic zebrafish also express GFP in olfactory neurons which we show represent an authentic rag1 expression site in zebrafish.
Errata / Notes
Ph.D. Thesis, Medical College of Georgia
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping