PUBLICATION
Molecular Embryology: Methods and Protocols
- Authors
- Sharpe, P.T. and Mason, I.
- ID
- ZDB-PUB-021016-126
- Date
- 1999
- Source
- Totowa, NJ: Humana Press : 756p (Book)
- Registered Authors
- Mason, Ivor
- Keywords
- Pt.1. The mouse embryo -- The mouse as a developmental model -- Culture of postimplantation mouse embryos -- Organ culture in the analysis of tissue interactions -- Treatment of mice with retinoids in vivo and in vitro: skeletal staining -- Cell grafting and labeling in postimplantation mouse embryos -- Production of transgenic rodents by the microinjection of coloned DNA into fertilized one-celled eggs -- Cre recombinase mediated alterations of the mouse genome using embryonic stem cells -- Gene trapping in mouse embryonic stem cells -- Production and growth of conditionally immortal cell lines from the H-2KbtsA58 transgenic mouse -- Reporter genes for the study of transcriptional regulation in transgenic mouse embryos -- Application of IacZ transgenic mice to cell lineage studies -- Mouse primordial germ cells: isolation and in vitro cultue -- Pt.2. The chicken embryo -- The avian embryo: an overview -- Chick embryos: incubation and isolation -- New culture -- Grafting Hensen's node -- Grafting of Somites -- Notochord grafts -- Transplantation of avian neural tissue -- Grafting of apical ridge and polarizing region --- Tissue recombinations in collagen gels -- Quail-chick chimeras -- Using fluorescent dyes for fate mapping, lineage analysis, and axon tracing in the chick embryo -- Pt.3. Amphibian embryo -- An overview of Xenopus development -- Mesoderm induction assays -- Experimental embryological methods for analysis of neural induction in the amphibian -- A method for generating transgenic frog embryos -- Axolotl/newt -- Pt.4. Zebrafish -- Pt.5. Nonvertebrate chordates -- Pt.6. Retroviruses -- Pt.7. Molecular techniques -- Pt.8. Microscopy and photography.
- MeSH Terms
- none
- PubMed
- none
Citation
Sharpe, P.T. and Mason, I. (1999) Molecular Embryology: Methods and Protocols. Totowa, NJ: Humana Press. :756p.
Abstract
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping