PUBLICATION
B cells develop in the zebrafish pancreas
- Authors
- Danilova, N. and Steiner, L.A.
- ID
- ZDB-PUB-021016-12
- Date
- 2002
- Source
- Proceedings of the National Academy of Sciences of the United States of America 99(21): 13711-13716 (Journal)
- Registered Authors
- Danilova, Nadia, Steiner, Lisa
- Keywords
- none
- MeSH Terms
-
- Animals
- B-Lymphocytes/cytology
- B-Lymphocytes/immunology*
- DNA/genetics
- Gene Expression Regulation, Developmental
- Gene Rearrangement, B-Lymphocyte
- Genes, RAG-1
- Immunoglobulin mu-Chains/genetics
- In Situ Hybridization
- Lymphopoiesis/genetics
- Lymphopoiesis/immunology
- Mice
- Pancreas/embryology*
- Pancreas/growth & development
- Pancreas/immunology*
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/growth & development
- Zebrafish/immunology*
- PubMed
- 12370418 Full text @ Proc. Natl. Acad. Sci. USA
Citation
Danilova, N. and Steiner, L.A. (2002) B cells develop in the zebrafish pancreas. Proceedings of the National Academy of Sciences of the United States of America. 99(21):13711-13716.
Abstract
The zebrafish, with its transparent free-living embryo, is a useful organism for investigating early stages in lymphopoiesis. Previously, we showed that T cells differentiate in the thymus by day 4, but no sites for B cell differentiation were seen until 3 weeks. We report here that on day 4, we detect rearrangements of genes encoding B cell receptors in DNA extracted from whole fish. Also by day 4, rag1 transcripts are seen in the pancreas, an organ not previously associated with lymphopoiesis; by day 10, Igmu; transcripts are detected here. Thus, in zebrafish, the pancreas assumes the role of both the liver in fetal mice and the spleen in neonatal mice.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping