PUBLICATION
A novel repressor-type homeobox gene, ved, is involved in dharma/bozozok-mediated dorsal organizer formation in zebrafish
- Authors
- Shimizu, T., Yamanaka, Y., Nojima, H., Yabe, T., Hibi, M., and Hirano, T.
- ID
- ZDB-PUB-021015-5
- Date
- 2002
- Source
- Mechanisms of Development 118(1-2): 125-38 (Journal)
- Registered Authors
- Hibi, Masahiko, Nojima, Hideaki, Shimizu, Takashi, Yabe, Taijirou, Yamanaka, Yojiro
- Keywords
- ved; dharma/bozozok; vox/vegal; vent/vega2; goosecoid; chordino; zebrafish
- MeSH Terms
-
- Transcriptional Activation
- Sequence Homology, Amino Acid
- Protein Structure, Tertiary
- Precipitin Tests
- Blotting, Western
- Amino Acid Sequence*
- RNA/metabolism
- Humans
- Amino Acid Motifs
- Plasmids/metabolism
- Herpes Simplex Virus Protein Vmw65/metabolism
- Zebrafish Proteins/biosynthesis
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/physiology*
- Transcription, Genetic
- Molecular Sequence Data
- Zebrafish
- Animals
- Cell Line
- Homeodomain Proteins/genetics*
- Homeodomain Proteins/physiology
- In Situ Hybridization
- Gene Expression Regulation, Developmental*
- Repressor Proteins/biosynthesis
- Repressor Proteins/genetics*
- Repressor Proteins/physiology*
- Phylogeny
- PubMed
- 12351176 Full text @ Mech. Dev.
Citation
Shimizu, T., Yamanaka, Y., Nojima, H., Yabe, T., Hibi, M., and Hirano, T. (2002) A novel repressor-type homeobox gene, ved, is involved in dharma/bozozok-mediated dorsal organizer formation in zebrafish. Mechanisms of Development. 118(1-2):125-38.
Abstract
Dharma/Bozozok (Dha/Boz) is a homeodomain protein containing an Engrailed homology (Eh) 1 repressor motif. It is important in zebrafish dorsal organizer formation. Dha/Boz interacted with a co-repressor Groucho through the Eh1 motif. Expression of a Dha/Boz fused to the transcriptional activator VP16 repressed dorsal axis formation and the expression of organizer genes but led to the dorsal expansion of expression of the homeobox gene vox/vega1, indicating that Dha/Boz functions as a transcriptional repressor for dorsal axis formation. We also isolated a novel homeobox gene, ved, whose expression was negatively regulated by dha/boz. ved's sequence and expression profile were similar to those of vox/vega1 and vent/vega2. Like Vox/Vega1 and Vent/Vega2, Ved acted as a transcriptional repressor. The combined inhibition of ved, vox/vega1, and vent/vega2, by antisense morpholino injection, strongly dorsalized the embryos and elicited ventral expansion of organizer gene expression, compared with the effect of inhibiting each of these genes alone. These results suggest that ved is a target for the repressor Dha/Boz. ved functions redundantly with vox/ vega1 and vent/vega2 to restrict the organizer domain.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping