PUBLICATION
Evidence that the separation of Mhc class II from class I loci in the zebrafish, Danio rerio, occurred by translocation
- Authors
- Kuroda, N., Figueroa, F., O'hUigin, C., and Klein, J.
- ID
- ZDB-PUB-021002-5
- Date
- 2002
- Source
- Immunogenetics 54(6): 418-430 (Journal)
- Registered Authors
- Figueroa, Felipe, Klein, Jan, O'hUigin, Colm
- Keywords
- major histocompatibility complex; Mhc class II genes; zebrafish Danio rerio; SLC7A4; amino acid transporter
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Chromosome Mapping
- Chromosomes, Artificial, P1 Bacteriophage/genetics
- DNA/genetics
- Evolution, Molecular
- Genes, MHC Class I*
- Genes, MHC Class II*
- Models, Genetic
- Molecular Sequence Data
- Multigene Family
- Open Reading Frames
- Phylogeny
- Sequence Homology, Amino Acid
- Sequence Homology, Nucleic Acid
- Translocation, Genetic
- Zebrafish/genetics*
- Zebrafish/immunology*
- PubMed
- 12242592 Full text @ Immunogenetics
Citation
Kuroda, N., Figueroa, F., O'hUigin, C., and Klein, J. (2002) Evidence that the separation of Mhc class II from class I loci in the zebrafish, Danio rerio, occurred by translocation. Immunogenetics. 54(6):418-430.
Abstract
In the zebrafish, Danio rerio, and other teleosts, the class I and class II loci of the major histocompatibility complex ( Mhc) reside on different chromosomes. To shed light on the events that might have generated this difference from tetrapods, in which these two types of loci are clustered in a single chromosomal region, the organization of the class II loci in linkage group 8 of the zebrafish was determined by the characterization of contigs of PAC clones. Three contigs were defined: DAB, DCB, and DBB. The 350-kb-long DAB contig contained only four genes: DDB, DAB, SLC7A4, and DAA. The 150-kb-long DCB contig contained the DCB, DCA, and fz10 genes at an undetermined distance from the DAB contig. And the 120-kb-long DBB contig comprised the DBB gene presumably in another linkage group. The low gene density of the linkage group 8 contigs, contrasting with the high gene density of the zebrafish class I region, and the close association with genes [ SLC7A4 coding for an amino acid transporter, and fz10 (frizzled 10) coding for a receptor of the WNT glycoprotein] that are not linked with the tetrapod Mhc, is interpreted to mean that the separation of the class II from class I loci in teleosts occurred by translocation rather than by genomic or chromosomal duplication.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping