PUBLICATION
Distribution of rod- and cone-specific phosducins in retinas of non-mammalian vertebrates
- Authors
- Kobayashi, Y., Hisatomi, O., Yamamoto, S., and Tokunaga, F.
- ID
- ZDB-PUB-020913-6
- Date
- 2002
- Source
- Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 133(1): 77-83 (Journal)
- Registered Authors
- Keywords
- Danio rerio; diurnal gecko; molecular evolution; Oryzias latipes; phosducin; photoreceptor cell; retina; teleost
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Blotting, Western
- Evolution, Molecular
- Eye Proteins/analysis*
- Eye Proteins/genetics
- Fishes
- GTP-Binding Protein Regulators
- Immunohistochemistry
- Molecular Sequence Data
- Organ Specificity
- Phosphoproteins/analysis*
- Phosphoproteins/genetics
- Phylogeny
- Retinal Cone Photoreceptor Cells/chemistry*
- Retinal Rod Photoreceptor Cells/chemistry*
- Sequence Alignment
- Zebrafish Proteins/analysis*
- PubMed
- 12223214 Full text @ Comp. Biochem. Physiol. B Biochem. Mol. Biol.
Citation
Kobayashi, Y., Hisatomi, O., Yamamoto, S., and Tokunaga, F. (2002) Distribution of rod- and cone-specific phosducins in retinas of non-mammalian vertebrates. Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology. 133(1):77-83.
Abstract
In mammalian retinas, it has been believed that just one kind of phosducin (PD) commonly exists in both rods and cones. However, we have previously reported that there are rod- and cone-specific PDs (OlPD-R and OlPD-C) in medaka (Oryzias latipes) retina [FEBS Lett., 502, 117-121, 2001]. To clarify the distribution and evolution of these photoreceptor type-specific PDs, we investigated PDs of another teleost and a reptile. Immunohistochemical and Western blot analyses using anti-medaka PD antisera demonstrated that two kinds of PDs are expressed in zebrafish (Danio rerio) photoreceptor cells. Our study is suggestive that teleosts generally possess rod- and cone-specific PDs. We isolated a cDNA encoding putative PD (PmlPD) of a diurnal gecko (Phelsuma madagascariensis longinsulae). Because diurnal gecko possesses a pure-cone retina, it was expected that PmlPD would be expressed in cones. Molecular phylogenetic analysis demonstrated that PmlPD was more closely related to mammalian PDs than teleost cone-specific PDs, suggesting that the rod- and cone-specific subtype of teleost PDs have arisen after the teleost-tetrapod divergence.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping